TY - JOUR
T1 - Nanosecond pulsed electric field (nsPEF) and vaccines
T2 - a novel technique for the inactivation of SARS-CoV-2 and other viruses?
AU - Ruiz-Fernández, A. R.
AU - Rosemblatt, M.
AU - Perez-Acle, T.
N1 - Funding Information:
The authors are pleased to acknowledge financial support from FONDECYT Iniciación 11221268 and from Cen-tro Ciencia & Vida, FB210008, Programa de Financiamiento Basal para Centros Cientificos y Tecnológicos de Excelencia de ANID. The authors acknowledge to Dr. Andrés Hojman for his suggestions to improve this article.
Publisher Copyright:
© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
PY - 2022
Y1 - 2022
N2 - Since the beginning of 2020, worldwide attention has been being focussed on SARS-CoV-2, the second strain of the severe acute respiratory syndrome virus. Although advances in vaccine technology have been made, particularly considering the advent of mRNA vaccines, up to date, no single antigen design can ensure optimal immune response. Therefore, new technologies must be tested as to their ability to further improve vaccines. Nanosecond Pulsed Electric Field (nsPEF) is one such method showing great promise in different biomedical and industrial fields, including the fight against COVID-19. Of note, available research shows that nsPEF directly damages the cell’s DNA, so it is critical to determine if this technology could be able to fragment either viral DNA or RNA so as to be used as a novel technology to produce inactivated pathogenic agents that may, in turn, be used for the production of vaccines. Considering the available evidence, we propose that nsPEF may be used to produce inactivated SARS-CoV-2 viruses that may in turn be used to produce novel vaccines, as another tool to address 20 the current COVID-19 pandemic.Key Messages Viral inactivation by using pulsed electric fields in the nanosecond frequency. DNA fragmentation by a Nanosecond Pulsed Electric Field (nsPEF). Opportunity to apply new technologies in vaccine development.
AB - Since the beginning of 2020, worldwide attention has been being focussed on SARS-CoV-2, the second strain of the severe acute respiratory syndrome virus. Although advances in vaccine technology have been made, particularly considering the advent of mRNA vaccines, up to date, no single antigen design can ensure optimal immune response. Therefore, new technologies must be tested as to their ability to further improve vaccines. Nanosecond Pulsed Electric Field (nsPEF) is one such method showing great promise in different biomedical and industrial fields, including the fight against COVID-19. Of note, available research shows that nsPEF directly damages the cell’s DNA, so it is critical to determine if this technology could be able to fragment either viral DNA or RNA so as to be used as a novel technology to produce inactivated pathogenic agents that may, in turn, be used for the production of vaccines. Considering the available evidence, we propose that nsPEF may be used to produce inactivated SARS-CoV-2 viruses that may in turn be used to produce novel vaccines, as another tool to address 20 the current COVID-19 pandemic.Key Messages Viral inactivation by using pulsed electric fields in the nanosecond frequency. DNA fragmentation by a Nanosecond Pulsed Electric Field (nsPEF). Opportunity to apply new technologies in vaccine development.
KW - NPS
KW - SARS-CoV-2
KW - nsPEF
KW - vaccines
UR - http://www.scopus.com/inward/record.url?scp=85133296639&partnerID=8YFLogxK
U2 - 10.1080/07853890.2022.2087898
DO - 10.1080/07853890.2022.2087898
M3 - Article
C2 - 35786157
AN - SCOPUS:85133296639
SN - 0785-3890
VL - 54
SP - 1749
EP - 1756
JO - Annals of Medicine
JF - Annals of Medicine
IS - 1
ER -