@inbook{05c220fe61934fdeb512216a0d324006,
title = "Transcriptomics using next generation sequencing technologies",
abstract = "Next generation sequencing technologies may now be applied to the study of transcriptomics. RNA-Seq or RNA sequencing employs high-throughput sequencing of complementary DNA fragments delivering a transcriptional profile. In this chapter, we aim to provide a starting point for Xenopus researchers planning on starting an RNA-Seq transcriptomics study. We begin by providing a section on template isolation and library preparation. The next section comprises the main bioinformatics procedures that need to be performed for raw data processing, normalization, and differential gene expression. Finally, we have included a section on studying deep sequencing results in Xenopus, which offers general guidance as to what can be done in this model.",
keywords = "High-throughput sequencing, Illumina, Massively -parallel sequencing, Next generation sequencing, RNA-Seq, Small RNA-Seq, Transcriptional profiling, Transcriptomics, Xenopus laevis, Xenopus tropicalis",
author = "Dasfne Lee-Liu and Almonacid, {Leonardo I.} and Fernando Faunes and Francisco Melo and Juan Larrain",
year = "2012",
doi = "10.1007/978-1-61779-992-1_18",
language = "English",
isbn = "9781617799914",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "293--317",
booktitle = "Xenopus Protocols",
}