TY - JOUR
T1 - Synthesis and expression of functional angiotensin II receptors in Xenopus oocytes injected with rat brain mRNA
AU - Cross, Daniel
AU - Cifuentes, Freddy
AU - Huidobro-Toro, J. Pablo
AU - Vío, Carlos P.
AU - Inestrosa, Nibaldo C.
N1 - Funding Information:
We would like to thank Drs. C. Allende and P. Carvallo for introducing us to the technique of the oocyte injection. We are also indebted to Dr. J. Szec6wka for helpful advice. This study was supported in part by Grants from the Stiftung Volkswa-genwerk, the Research Direction of the Catholic University and from the Gildemeister Foundation to N. C.I. and Dr. Jaime Alvarez.
PY - 1987/9
Y1 - 1987/9
N2 - Xenopus laevis oocytes were injected with poly(A)+ mRNA isolated from rat brain and superfused in a medium containing either serotonin, angiotensin II or bradykinin. Applications of serotonin or angiotensin II to injected oocytes elicited, in a dose-dependent manner, changes in membrane potential. The angiotensin II receptor was desensitized fairly rapidly in the continued presence of the agonist. No response was obtained with bradykinin. The selectivity of the angiotensin II-induced response was demonstrated by the finding that the angiotensin II antagonist ([Sar1,Ala8]angiotensin II, saralasin) blocked the angiotensin II-induced response. It is concluded that an appropriate fraction of brain mRNA is capable of directing the synthesis and correct insertion of functional angiotensin II receptors in the Xenopus oocyte membrane.
AB - Xenopus laevis oocytes were injected with poly(A)+ mRNA isolated from rat brain and superfused in a medium containing either serotonin, angiotensin II or bradykinin. Applications of serotonin or angiotensin II to injected oocytes elicited, in a dose-dependent manner, changes in membrane potential. The angiotensin II receptor was desensitized fairly rapidly in the continued presence of the agonist. No response was obtained with bradykinin. The selectivity of the angiotensin II-induced response was demonstrated by the finding that the angiotensin II antagonist ([Sar1,Ala8]angiotensin II, saralasin) blocked the angiotensin II-induced response. It is concluded that an appropriate fraction of brain mRNA is capable of directing the synthesis and correct insertion of functional angiotensin II receptors in the Xenopus oocyte membrane.
KW - Angiotensin II receptor
KW - Brain mRNA
KW - Microinjection
KW - Saralasin
KW - Xenopus laevis oocyte
UR - http://www.scopus.com/inward/record.url?scp=0023247146&partnerID=8YFLogxK
U2 - 10.1016/0169-328X(87)90034-9
DO - 10.1016/0169-328X(87)90034-9
M3 - Article
C2 - 3676711
AN - SCOPUS:0023247146
SN - 0169-328X
VL - 2
SP - 268
EP - 270
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 3
ER -