TY - JOUR
T1 - Single-cell imaging tools for brain energy metabolism
T2 - A review
AU - San Martín, Alejandro
AU - Sotelo-Hitschfeld, Tamara
AU - Lerchundi, Rodrigo
AU - Fernández-Moncada, Ignacio
AU - Ceballo, Sebastian
AU - Valdebenito, Rocío
AU - Baeza-Lehnert, Felipe
AU - Alegría, Karin
AU - Contreras-Baeza, Yasna
AU - Garrido-Gerter, Pamela
AU - Romero-Gómez, Ignacio
AU - Barros, L. Felipe
N1 - Publisher Copyright:
© The Authors.
PY - 2014/7/1
Y1 - 2014/7/1
N2 - Neurophotonics comes to light at a time in which advances in microscopy and improved calcium reporters are paving the way toward high-resolution functional mapping of the brain. This review relates to a parallel revolution in metabolism. We argue that metabolism needs to be approached both in vitro and in vivo, and that it does not just exist as a low-level platform but is also a relevant player in information processing. In recent years, genetically encoded fluorescent nanosensors have been introduced to measure glucose, glutamate, ATP, NADH, lactate, and pyruvate in mammalian cells. Reporting relative metabolite levels, absolute concentrations, and metabolic fluxes, these sensors are instrumental for the discovery of new molecular mechanisms. Sensors continue to be developed, which together with a continued improvement in protein expression strategies and new imaging technologies, herald an exciting era of high-resolution characterization of metabolism in the brain and other organs.
AB - Neurophotonics comes to light at a time in which advances in microscopy and improved calcium reporters are paving the way toward high-resolution functional mapping of the brain. This review relates to a parallel revolution in metabolism. We argue that metabolism needs to be approached both in vitro and in vivo, and that it does not just exist as a low-level platform but is also a relevant player in information processing. In recent years, genetically encoded fluorescent nanosensors have been introduced to measure glucose, glutamate, ATP, NADH, lactate, and pyruvate in mammalian cells. Reporting relative metabolite levels, absolute concentrations, and metabolic fluxes, these sensors are instrumental for the discovery of new molecular mechanisms. Sensors continue to be developed, which together with a continued improvement in protein expression strategies and new imaging technologies, herald an exciting era of high-resolution characterization of metabolism in the brain and other organs.
KW - Förster resonance energy transfer
KW - glycolysis
KW - membrane transport
KW - metabolic flux
KW - mitochondria
KW - optogenetics
UR - http://www.scopus.com/inward/record.url?scp=84978942575&partnerID=8YFLogxK
U2 - 10.1117/1.NPh.1.1.011004
DO - 10.1117/1.NPh.1.1.011004
M3 - Review article
AN - SCOPUS:84978942575
SN - 2329-423X
VL - 1
JO - Neurophotonics
JF - Neurophotonics
IS - 1
M1 - 011004
ER -