Single-cell imaging tools for brain energy metabolism: A review

Alejandro San Martín, Tamara Sotelo-Hitschfeld, Rodrigo Lerchundi, Ignacio Fernández-Moncada, Sebastian Ceballo, Rocío Valdebenito, Felipe Baeza-Lehnert, Karin Alegría, Yasna Contreras-Baeza, Pamela Garrido-Gerter, Ignacio Romero-Gómez, L. Felipe Barros*

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículo de revisiónrevisión exhaustiva

50 Citas (Scopus)

Resumen

Neurophotonics comes to light at a time in which advances in microscopy and improved calcium reporters are paving the way toward high-resolution functional mapping of the brain. This review relates to a parallel revolution in metabolism. We argue that metabolism needs to be approached both in vitro and in vivo, and that it does not just exist as a low-level platform but is also a relevant player in information processing. In recent years, genetically encoded fluorescent nanosensors have been introduced to measure glucose, glutamate, ATP, NADH, lactate, and pyruvate in mammalian cells. Reporting relative metabolite levels, absolute concentrations, and metabolic fluxes, these sensors are instrumental for the discovery of new molecular mechanisms. Sensors continue to be developed, which together with a continued improvement in protein expression strategies and new imaging technologies, herald an exciting era of high-resolution characterization of metabolism in the brain and other organs.

Idioma originalInglés
Número de artículo011004
PublicaciónNeurophotonics
Volumen1
N.º1
DOI
EstadoPublicada - 2014
Publicado de forma externa

Nota bibliográfica

Publisher Copyright:
© The Authors.

Áreas temáticas de ASJC Scopus

  • Neurociencia (miscelánea)
  • Tecnología radiológica y de ultrasonidos
  • Radiología, medicina nuclear y obtención de imágenes

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