Single-cell CNV detection in human neuronal nuclei

Margaret B. Wierman; Ian E. Burbulis; William D. Chronister; Stefan Bekiranov; Michael J. McConnell

doi:10.1007/978-1-4939-7280-7_6

Single-cell CNV detection in human neuronal nuclei

Margaret B. Wierman, Ian E. Burbulis, William D. Chronister, Stefan Bekiranov, Michael J. McConnell^*

^*Autor correspondiente de este trabajo

University of Virginia

Producción científica: Capítulo del libro/informe/acta de congreso › Capítulo › revisión exhaustiva

3 Citas (Scopus)

Resumen

Genomic mosaicism is prevalent throughout human somatic tissues and is much more common than previously thought. Here, we describe step-by-step methods to isolate neuronal nuclei from human brain and identify megabase-scale copy number variants (CNVs) in single nuclei. The approach detailed herein includes use of CellRaft technology for single-nucleus isolation, the PicoPLEX approach to whole-genome amplification and library preparation, and a pooled library purification protocol, termed Gel2Gel, which has been developed in our laboratory. These methods are focused toward neuroscience research, but are adaptable to many biomedical fields.

Idioma original	Inglés
Título de la publicación alojada	Neuromethods
Editorial	Humana Press Inc.
Páginas	109-131
Número de páginas	23
DOI	https://doi.org/10.1007/978-1-4939-7280-7_6
Estado	Publicada - 2017

Serie de la publicación

Nombre	Neuromethods
Volumen	131
ISSN (versión impresa)	0893-2336
ISSN (versión digital)	1940-6045

Nota bibliográfica

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.

Áreas temáticas de ASJC Scopus

Neurociencias General
Bioquímica, Genética y Biología Molecular General
Farmacología, toxicología y ciencias farmacéuticas (todo)
Psiquiatría y salud mental

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Acceder al documento

10.1007/978-1-4939-7280-7_6

Otros archivos y enlaces

Enlace a la publicación en Scopus

Citar esto

@inbook{03812d714103406399fe68c09646472b,

title = "Single-cell CNV detection in human neuronal nuclei",

abstract = "Genomic mosaicism is prevalent throughout human somatic tissues and is much more common than previously thought. Here, we describe step-by-step methods to isolate neuronal nuclei from human brain and identify megabase-scale copy number variants (CNVs) in single nuclei. The approach detailed herein includes use of CellRaft technology for single-nucleus isolation, the PicoPLEX approach to whole-genome amplification and library preparation, and a pooled library purification protocol, termed Gel2Gel, which has been developed in our laboratory. These methods are focused toward neuroscience research, but are adaptable to many biomedical fields.",

keywords = "And somatic mosaicism, Copy number variation, Neurons, Single-cell genome sequencing, Whole-genome amplification",

author = "Wierman, {Margaret B.} and Burbulis, {Ian E.} and Chronister, {William D.} and Stefan Bekiranov and McConnell, {Michael J.}",

note = "Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media LLC.",

year = "2017",

doi = "10.1007/978-1-4939-7280-7_6",

language = "English",

series = "Neuromethods",

publisher = "Humana Press Inc.",

pages = "109--131",

booktitle = "Neuromethods",

}

TY - CHAP

T1 - Single-cell CNV detection in human neuronal nuclei

AU - Wierman, Margaret B.

AU - Burbulis, Ian E.

AU - Chronister, William D.

AU - Bekiranov, Stefan

AU - McConnell, Michael J.

PY - 2017

Y1 - 2017

N2 - Genomic mosaicism is prevalent throughout human somatic tissues and is much more common than previously thought. Here, we describe step-by-step methods to isolate neuronal nuclei from human brain and identify megabase-scale copy number variants (CNVs) in single nuclei. The approach detailed herein includes use of CellRaft technology for single-nucleus isolation, the PicoPLEX approach to whole-genome amplification and library preparation, and a pooled library purification protocol, termed Gel2Gel, which has been developed in our laboratory. These methods are focused toward neuroscience research, but are adaptable to many biomedical fields.

AB - Genomic mosaicism is prevalent throughout human somatic tissues and is much more common than previously thought. Here, we describe step-by-step methods to isolate neuronal nuclei from human brain and identify megabase-scale copy number variants (CNVs) in single nuclei. The approach detailed herein includes use of CellRaft technology for single-nucleus isolation, the PicoPLEX approach to whole-genome amplification and library preparation, and a pooled library purification protocol, termed Gel2Gel, which has been developed in our laboratory. These methods are focused toward neuroscience research, but are adaptable to many biomedical fields.

KW - And somatic mosaicism

KW - Copy number variation

KW - Neurons

KW - Single-cell genome sequencing

KW - Whole-genome amplification

UR - http://www.scopus.com/inward/record.url?scp=85029354537&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-7280-7_6

DO - 10.1007/978-1-4939-7280-7_6

M3 - Chapter

AN - SCOPUS:85029354537

T3 - Neuromethods

SP - 109

EP - 131

BT - Neuromethods

PB - Humana Press Inc.

ER -

Single-cell CNV detection in human neuronal nuclei

Resumen

Serie de la publicación

Nota bibliográfica

Áreas temáticas de ASJC Scopus

ODS de las Naciones Unidas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto