TY - JOUR
T1 - PTMs on H3 Variants before Chromatin Assembly Potentiate Their Final Epigenetic State
AU - Loyola, Alejandra
AU - Bonaldi, Tiziana
AU - Roche, Danièle
AU - Imhof, Axel
AU - Almouzni, Geneviève
N1 - Funding Information:
We dedicate this work to Wolfram Hörz: a great scientist, mentor, and friend. We thank H. Tagami and Y. Nakatani for Flag/HA H3.1 and H3.3 cells, and antibodies (G9a and Eu-HMTase); D. Schultz for SetDB1 baculovirus; T. Jenuwein for recombinant SUV39H1, wild-type, and Suv39h dn MEFs; A. Bardin, S. Barton, P. Becker, P.-A. Defossez, S. Dent, E. Heard, A. Groth, and J.-P. Quivy for comments; and T. Straub for bioinformatic support. A.L. and T.B. are supported by EMBO long-term fellowships. G.A.'s team is supported by la Ligue Nationale contre le Cancer, the Commissariat à l'Energie Atomique, European Contract RTN, Collaborative Programme between the Curie Institute and the Commissariat à l'Energie Atomique, Network of Excellence Epigenome, Cancéropôle Ile-de-France, and ACI-DRAB. A.I.'s team is supported by the Deutsche Forschungsgemeinschaft.
PY - 2006/10/20
Y1 - 2006/10/20
N2 - Histone posttranslational modifications (PTMs) and sequence variants regulate genome function. Although accumulating evidence links particular PTM patterns with specific genomic loci, our knowledge concerning where and when these PTMs are imposed remains limited. Here, we find that lysine methylation is absent prior to histone incorporation into chromatin, except at H3K9. Nonnucleosomal H3.1 and H3.3 show distinct enrichments in H3K9me, such that H3.1 contains more K9me1 than H3.3. In addition, H3.3 presents other modifications, including K9/K14 diacetylated and K9me2. Importantly, H3K9me3 was undetectable in both nonnucleosomal variants. Notably, initial modifications on H3 variants can potentiate the action of enzymes as exemplified with Suv39HMTase to produce H3K9me3 as found in pericentric heterochromatin. Although the set of initial modifications present on H3.1 is permissive for further modifications, in H3.3 a subset cannot be K9me3. Thus, initial modifications impact final PTMs within chromatin.
AB - Histone posttranslational modifications (PTMs) and sequence variants regulate genome function. Although accumulating evidence links particular PTM patterns with specific genomic loci, our knowledge concerning where and when these PTMs are imposed remains limited. Here, we find that lysine methylation is absent prior to histone incorporation into chromatin, except at H3K9. Nonnucleosomal H3.1 and H3.3 show distinct enrichments in H3K9me, such that H3.1 contains more K9me1 than H3.3. In addition, H3.3 presents other modifications, including K9/K14 diacetylated and K9me2. Importantly, H3K9me3 was undetectable in both nonnucleosomal variants. Notably, initial modifications on H3 variants can potentiate the action of enzymes as exemplified with Suv39HMTase to produce H3K9me3 as found in pericentric heterochromatin. Although the set of initial modifications present on H3.1 is permissive for further modifications, in H3.3 a subset cannot be K9me3. Thus, initial modifications impact final PTMs within chromatin.
KW - DNA
UR - https://www.scopus.com/pages/publications/33749657892
U2 - 10.1016/j.molcel.2006.08.019
DO - 10.1016/j.molcel.2006.08.019
M3 - Article
C2 - 17052464
AN - SCOPUS:33749657892
SN - 1097-2765
VL - 24
SP - 309
EP - 316
JO - Molecular Cell
JF - Molecular Cell
IS - 2
ER -