Production and biotransformation of phytosterol microdispersions to produce 4-androstene-3,17-dione

Rodrigo A. Mancilla; Roberto Pavez-Díaz; Alejandro Amoroso

doi:10.1007/978-1-4939-7183-1_11

Production and biotransformation of phytosterol microdispersions to produce 4-androstene-3,17-dione

Rodrigo A. Mancilla^*, Roberto Pavez-Díaz, Alejandro Amoroso

^*Autor correspondiente de este trabajo

Universidad San Sebastián

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3 Citas (Scopus)

Resumen

The current state of knowledge regarding phytosterols biotransformation to produce the steroid intermediate 4-androstene-3,17-dione (AD) shows different technologies. However, the initial concentration of phytosterols in batch cultures is limited due to its low solubility in aqueous media, causing serious difficulties for scaling up because of the low mass transfer. In this chapter, we describe a fermentation method of a phytosterol microdispersion with Mycobacterium sp. B3805 in the context of an integral technology for AD production. The microdispersion generation is based on a patent application that claims the production of an aqueous phytosterol microdispersion with an average size particle of 370 nm, and high stability and solubility in water at high phytosterols concentrations (Harting et al., 2012/US0046254). Our results indicate that up to 20 g/L phytosterols can be biotransformed with this technology allowing a good dispersion and stability of reactants in the fermentation broth.

Idioma original	Inglés
Título de la publicación alojada	Methods in Molecular Biology
Editorial	Humana Press Inc.
Páginas	159-165
Número de páginas	7
DOI	https://doi.org/10.1007/978-1-4939-7183-1_11
Estado	Publicada - 2017

Serie de la publicación

Nombre	Methods in Molecular Biology
Volumen	1645
ISSN (versión impresa)	1064-3745

Nota bibliográfica

Funding Information:
This work was supported by Grant N° 12CTI-16838 from CORFO and by Grant operational expenses N°21110224 from Conicyt. R.A. Mancilla was a doctoral fellow supported by CONICYT-Chile. The authors would like to thank Msc Raúl Aravena from Nutrartis S.A. for his support formulating dispersions, Dr. Cecilia Brañez from Naturalis S.A. for conceptual contribution to the project, and Karen Marquez and Camila Ahumada from PUCV research staff for technical assistance.

Publisher Copyright:
© Springer Science+Business Media LLC 2017.

Áreas temáticas de ASJC Scopus

Biología molecular
Genética

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abstract = "The current state of knowledge regarding phytosterols biotransformation to produce the steroid intermediate 4-androstene-3,17-dione (AD) shows different technologies. However, the initial concentration of phytosterols in batch cultures is limited due to its low solubility in aqueous media, causing serious difficulties for scaling up because of the low mass transfer. In this chapter, we describe a fermentation method of a phytosterol microdispersion with Mycobacterium sp. B3805 in the context of an integral technology for AD production. The microdispersion generation is based on a patent application that claims the production of an aqueous phytosterol microdispersion with an average size particle of 370 nm, and high stability and solubility in water at high phytosterols concentrations (Harting et al., 2012/US0046254). Our results indicate that up to 20 g/L phytosterols can be biotransformed with this technology allowing a good dispersion and stability of reactants in the fermentation broth.",

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Production and biotransformation of phytosterol microdispersions to produce 4-androstene-3,17-dione. / Mancilla, Rodrigo A.; Pavez-Díaz, Roberto; Amoroso, Alejandro.
Methods in Molecular Biology. Humana Press Inc., 2017. p. 159-165 (Methods in Molecular Biology; Vol. 1645).

Producción científica: Capítulo del libro/informe/acta de congreso › Capítulo › revisión exhaustiva

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AU - Pavez-Díaz, Roberto

AU - Amoroso, Alejandro

N1 - Publisher Copyright: © Springer Science+Business Media LLC 2017.

PY - 2017

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Production and biotransformation of phytosterol microdispersions to produce 4-androstene-3,17-dione

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