Phagocytosis of Leptospira by leukocytes from mice with different susceptibility to leptospirosis and possible role of chemokines

Paloma L.Da Silva, Fabiana Lauretti-Ferreira, Maiara Caldas De Lima, Swiany Silveira Lima, Ambart E. Covarrubias, Marcelo De Franco, Eneas Carvalho, Paulo Lee Ho, Renata M.A. Da Costa, Elizabeth A.L. Martins, Josefa B. Da Silva*

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

3 Citas (Scopus)

Resumen

Background: Leptospirosis is a widespread zoonosis caused by pathogenic prokaryotic microbes of the genus Leptospira. Although there are several reports in the literature, host-pathogen interaction is still poorly understood. The role of chemokine expression is important on the chemotaxis, activation and regulation of immune cells. Recent studies have shown that their expression profiles play an important role on the severity of leptospirosis outcome. We evaluated the phagocytosis of Leptospira by spleens cells from C3H/HeJ, C3H/HePas and BALB/c mouse strains, respectively susceptible, intermediate and resistant to leptospirosis, and by RAW 264.7 macrophages. Besides, we evaluated the effects of CCL2 treatment on the phagocytosis. The cells were incubated with or without CCL2 chemokine, and infected with virulent L. interrogans sv Copenhageni. Cells and culture supernatants were collected for subsequent analysis. Results: The number of leptospires was higher in BALB/c cells, CCL2 pre-treated or only infected groups, when compared to C3H/HeJ and C3H/HePas cells. Indeed, CCL2 activation did not interfere in the phagocytosis of Leptospira. Expression of chemokines CXCL5 and CCL8 levels were significantly inhibited in infected BALB/c cells when compared to the non-infected control. Conclusions: Higher ability to phagocytosis and early modulation of some chemokines correlated with the resistance to leptospirosis disease. Exposure to CCL2 did not interfere on phagocytosis of Leptospira in our experimental conditions, but acted in the modulation of chemokines expression during Leptospira infection.

Idioma originalInglés
Número de artículo4
PublicaciónBMC Microbiology
Volumen19
N.º1
DOI
EstadoPublicada - 2019

Nota bibliográfica

Funding Information:
This work received grants from Fundação de Amparo a Pesquisa do Estado de São Paulo-FAPESP (JBS grant 2013/16451–1), Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (JBS grant 473035/2013–8), and Fundação Butantan (Brazil). PLDS received scholarship from Instituto Butantan-Secretaria da Saúde do Estado de São Paulo and additional regular support from Fundação Butantan. MC received scholarship from CNPq and additional regular support from Fundação Butantan. Funding bodies did not have any role in study design, data collection and analysis, decision to publish, or writing the manuscript.

Publisher Copyright:
© 2019 The Author(s).

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Microbiología (médica)

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