TY - JOUR
T1 - Mesangial cells are able to produce catecholamines in vitro
AU - Di Marco, Giovana Seno
AU - Naffah-Mazzacoratti, Maria da Graça
AU - Vio, Carlos P.
AU - Pavão Dos Santos, Oscar Fernando
AU - Schor, Nestor
AU - Casarini, Dulce Elena
PY - 2003/5/1
Y1 - 2003/5/1
N2 - Mesangial cells (MC) participate in the control of the glomerular function due to their ability to synthesize hormones and induce cell contraction. Since MC can produce various kinds of hormones, the purpose of the present study was to determine if they are able to synthesize catecholamines. For this evaluation, the levels of norepinephrine, epinephrine, dopamine, and biopterin, the enzymatic cofactor of tyrosine hydroxylase (TH), were analyzed by HPLC in the intracellular compartment and in the medium of primary cultured MC. To identify and locate the enzymes responsible for monoamine synthesis, TH, dopa decarboxylase, and dopamine β-hydroxylase, Western blotting and immunocytochemistry were employed using monoclonal and polyclonal antibodies. Concentrations of NE=57±8, EPI=82±10, and DA=52±9 pg/mg protein (X±SEM) were found in the cell homogenate. The culture medium showed concentrations of NE=25±3, EPI=33±3, and DA=62± 15 pg/mg protein. Western blotting analysis and immunocytochemistry evidenced the presence of all enzymes. Moreover, biopterin was also detected in the intracellular compartment and in the medium (0.28±0.03 and 5.70±2 nmol/mg cell protein, respectively). Overall, the data indicate that MC have the biosynthetic machinery necessary to produce catecholamines, suggesting that they can act as a paracrine/autocrine hormone system, contributing to the regulation of glomerular hemodynamic and renal microcirculation.
AB - Mesangial cells (MC) participate in the control of the glomerular function due to their ability to synthesize hormones and induce cell contraction. Since MC can produce various kinds of hormones, the purpose of the present study was to determine if they are able to synthesize catecholamines. For this evaluation, the levels of norepinephrine, epinephrine, dopamine, and biopterin, the enzymatic cofactor of tyrosine hydroxylase (TH), were analyzed by HPLC in the intracellular compartment and in the medium of primary cultured MC. To identify and locate the enzymes responsible for monoamine synthesis, TH, dopa decarboxylase, and dopamine β-hydroxylase, Western blotting and immunocytochemistry were employed using monoclonal and polyclonal antibodies. Concentrations of NE=57±8, EPI=82±10, and DA=52±9 pg/mg protein (X±SEM) were found in the cell homogenate. The culture medium showed concentrations of NE=25±3, EPI=33±3, and DA=62± 15 pg/mg protein. Western blotting analysis and immunocytochemistry evidenced the presence of all enzymes. Moreover, biopterin was also detected in the intracellular compartment and in the medium (0.28±0.03 and 5.70±2 nmol/mg cell protein, respectively). Overall, the data indicate that MC have the biosynthetic machinery necessary to produce catecholamines, suggesting that they can act as a paracrine/autocrine hormone system, contributing to the regulation of glomerular hemodynamic and renal microcirculation.
KW - Biopterin
KW - Catecholamine production
KW - Mesangial cells
KW - Tyrosine hydroxylase
UR - http://www.scopus.com/inward/record.url?scp=0344921337&partnerID=8YFLogxK
U2 - 10.1002/jcb.10485
DO - 10.1002/jcb.10485
M3 - Article
C2 - 12682915
AN - SCOPUS:0344921337
SN - 0730-2312
VL - 89
SP - 144
EP - 151
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
IS - 1
ER -