Membrane vesicles in Acidithiobacillia class extreme acidophiles: influence on collective behaviors of ‘Fervidacidithiobacillus caldus’

Stefano Rossoni, Simón Beard, María Ignacia Segura-Bidermann, Juan Duarte-Ramírez, Francisco Kirhman Osorio, Manuel Varas-Godoy, Patricio Martínez-Bellange, Mario Vera, Raquel Quatrini*, Matías Castro*

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

1 Cita (Scopus)

Resumen

Membrane vesicles (MVs) are envelope-derived extracellular sacs that perform a broad diversity of physiological functions in bacteria. While considerably studied in pathogenic microorganisms, the roles, relevance, and biotechnological potential of MVs from environmental bacteria are less well established. Acidithiobacillaceae family bacteria are active players in the sulfur and iron biogeochemical cycles in extremely acidic environments and drivers of the leaching of mineral ores contributing to acid rock/mine drainage (ARD/AMD) and industrial bioleaching. One key aspect of such a role is the ability of these bacteria to tightly interact with the mineral surfaces and extract electrons and nutrients to support their chemolithotrophic metabolism. Despite recent advances in the characterization of acidithiobacilli biofilms and extracellular matrix (ECM) components, our understanding of its architectural and mechanistic aspects remains scant. Using different microscopy techniques and nano-tracking analysis we show that vesiculation is a common phenomenon in distant members of the Acidithiobacillaceae family, and further explore the role of MVs in multicellular colonization behaviors using ‘Fervidacidithiobacillus caldus’ as a bacterial model. Production of MVs in ‘F. caldus’ occurred in both planktonic cultures and biofilms formed on sulfur surfaces, where MVs appeared individually or in chains resembling tube-shaped membranous structures (TSMSs) important for microbial communication. Liquid chromatography–mass spectrometry data and bioinformatic analysis of the MV-associated proteome revealed that ‘F. caldus’ MVs were enriched in proteins involved in cell–cell and cell–surface processes and largely typified the MVs as outer MVs (OMVs). Finally, microbiological assays showed that amendment of ‘F. caldus’ MVs to cells and/or biofilms affects collective colonizing behaviors relevant to the ecophysiology and applications of these acidophiles, providing grounds for their exploitation in biomining.

Idioma originalInglés
Número de artículo1331363
PublicaciónFrontiers in Microbiology
Volumen14
DOI
EstadoPublicada - 2023

Nota bibliográfica

Publisher Copyright:
Copyright © 2024 Rossoni, Beard, Segura-Bidermann, Duarte-Ramírez, Osorio, Varas-Godoy, Martínez-Bellange, Vera, Quatrini and Castro.

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Microbiología (médica)

Huella

Profundice en los temas de investigación de 'Membrane vesicles in Acidithiobacillia class extreme acidophiles: influence on collective behaviors of ‘Fervidacidithiobacillus caldus’'. En conjunto forman una huella única.

Citar esto