Hantavirus Gn and Gc glycoproteins self-assemble into virus-like particles

Rodrigo Acuña; Nicolás Cifuentes-Muñoz; Chantal L. Márquez; Manuela Bulling; Jonas Klingström; Roberta Mancini; Pierre Yves Lozach; Nicole D. Tischlera

doi:10.1128/JVI.03118-13

Hantavirus Gn and Gc glycoproteins self-assemble into virus-like particles

Rodrigo Acuña, Nicolás Cifuentes-Muñoz, Chantal L. Márquez, Manuela Bulling, Jonas Klingström, Roberta Mancini, Pierre Yves Lozach, Nicole D. Tischlera^*

^*Autor correspondiente de este trabajo

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39 Citas (Scopus)

Resumen

How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.

Idioma original	Inglés
Páginas (desde-hasta)	2344-2348
Número de páginas	5
Publicación	Journal of Virology
Volumen	88
N.º	4
DOI	https://doi.org/10.1128/JVI.03118-13
Estado	Publicada - 2014
Publicado de forma externa	Sí

Áreas temáticas de ASJC Scopus

Microbiología
Inmunología
Insectos
Virología

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10.1128/JVI.03118-13

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abstract = "How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.",

author = "Rodrigo Acu{\~n}a and Nicol{\'a}s Cifuentes-Mu{\~n}oz and M{\'a}rquez, {Chantal L.} and Manuela Bulling and Jonas Klingstr{\"o}m and Roberta Mancini and Lozach, {Pierre Yves} and Tischlera, {Nicole D.}",

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AU - Acuña, Rodrigo

AU - Cifuentes-Muñoz, Nicolás

AU - Márquez, Chantal L.

AU - Bulling, Manuela

AU - Klingström, Jonas

AU - Mancini, Roberta

AU - Lozach, Pierre Yves

AU - Tischlera, Nicole D.

PY - 2014/2

Y1 - 2014/2

N2 - How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.

AB - How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.

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JF - Journal of Virology

IS - 4

ER -

Hantavirus Gn and Gc glycoproteins self-assemble into virus-like particles

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