Gold nanoparticles as tracking devices to shed light on the role of caveolin-1 in early stages of melanoma metastasis

Simón Guerrero, Victor Manuel Díaz-García, Pamela Contreras-Orellana, Pablo Lara, Sujey Palma, Fanny Guzman, Lorena Lobos-Gonzalez, Areli Cárdenas, Ximena Rojas-Silva, Luis Muñoz, Lisette Leyton, Marcelo J. Kogan, Andrew Fg Quest*

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

8 Citas (Scopus)

Resumen

Aim: To track early events during lung metastasis, we labeled cells expressing (B16F10CAV1) or lacking CAV1 (B16F10mock) with gold nanoparticles conjugated to the peptide TAT (AuNPs-PEG-TAT). Methods: B16F10 expressing or lacking CAV1 were labeled with AuNPs-PEG-TAT. The physicochemical properties and cytotoxicity of these nanoparticles, as well as their effects on migration and invasiveness of B16F10 cells in vitro were evaluated. Ex vivo lung distribution of the labeled cells after tail vein injection into C57BL/6 mice was examined. Results: AuNPs-PEG-TAT did not affect B16F10 viability, migration and invasiveness. The metastatic and tumorigenic capability of the labeled B16F10 was also not modified in comparison to unlabeled B16F10 cells. CAV1 expression favored the retention of B16F10 cells in the lungs of mice 2 h post injection, suggesting CAV1 promoted adherence to endothelial cells and transendothelial migration. Conclusions: We developed a protocol to label B16F10 cells with AuNPs-PEG-TAT that permits subsequent tracking of cells in mice. CAV1 overexpression was found to increase retention and transendothelial migration of B16F10 cells in the lung.

Idioma originalInglés
Páginas (desde-hasta)1447-1462
Número de páginas16
PublicaciónNanomedicine
Volumen13
N.º12
DOI
EstadoPublicada - 2018

Nota bibliográfica

Publisher Copyright:
© 2018 2018 Future Medicine Ltd.

Áreas temáticas de ASJC Scopus

  • Bioingeniería
  • Desarrollo
  • Ingeniería biomédica
  • Ciencia de los Materiales General
  • Medicina (miscelánea)

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