Expression of Aeromonas caviae ST pyruvate dehydrogenase complex components mediate tellurite resistance in Escherichia coli

Miguel E. Castro, Roberto C. Molina, Waldo A. Díaz, Gonzalo A. Pradenas, Claudio C. Vásquez*

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

24 Citas (Scopus)

Resumen

Potassium tellurite (K2TeO3) is harmful to most organisms and specific mechanisms explaining its toxicity are not well known to date. We previously reported that the lpdA gene product of the tellurite-resistant environmental isolate Aeromonas caviae ST is involved in the reduction of tellurite to elemental tellurium. In this work, we show that expression of A. caviae ST aceE, aceF, and lpdA genes, encoding pyruvate dehydrogenase, dihydrolipoamide transacetylase, and dihydrolipoamide dehydrogenase, respectively, results in tellurite resistance and decreased levels of tellurite-induced superoxide in Escherichia coli. In addition to oxidative damage resulting from tellurite exposure, a metabolic disorder would be simultaneously established in which the pyruvate dehydrogenase complex would represent an intracellular tellurite target. These results allow us to widen our vision regarding the molecular mechanisms involved in bacterial tellurite resistance by correlating tellurite toxicity and key enzymes of aerobic metabolism.

Idioma originalInglés
Páginas (desde-hasta)148-152
Número de páginas5
PublicaciónBiochemical and Biophysical Research Communications
Volumen380
N.º1
DOI
EstadoPublicada - 2009
Publicado de forma externa

Áreas temáticas de ASJC Scopus

  • Biofísica
  • Bioquímica
  • Biología molecular
  • Biología celular

Huella

Profundice en los temas de investigación de 'Expression of Aeromonas caviae ST pyruvate dehydrogenase complex components mediate tellurite resistance in Escherichia coli'. En conjunto forman una huella única.

Citar esto