Expression and subcellular localization of mouse 20S proteasome activator complex PA28

Andrea Soza; Christine Knuehl; Marcus Groettrup; Peter Henklein; Keiji Tanaka; Peter M. Kloetzel

doi:10.1016/S0014-5793(97)00864-8

Expression and subcellular localization of mouse 20S proteasome activator complex PA28

Andrea Soza, Christine Knuehl, Marcus Groettrup, Peter Henklein, Keiji Tanaka, Peter M. Kloetzel^*

^*Autor correspondiente de este trabajo

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

59 Citas (Scopus)

Resumen

We have cloned the mouse PA28 proteasome activator cDNAs. Northern blot demonstrates high PA28 mRNA levels in liver, kidney and lung, mRNA levels are low in thymus, spleen and brain. In contrast, PA28 protein levels vary little between these tissues. Immunocytological analysis and cell fractionation experiments demonstrate that both subunits are almost equally distributed between the cytoplasm and the nucleus. Interestingly, PA28α spares nucleoli, while PA28β is strongly enhanced in the nucleolus. This indicates for the first time that the PA28α and PA28β subunits may serve nuclear functions which may be different from and independent of each other.

Idioma original	Inglés
Páginas (desde-hasta)	27-34
Número de páginas	8
Publicación	FEBS Letters
Volumen	413
N.º	1
DOI	https://doi.org/10.1016/S0014-5793(97)00864-8
Estado	Publicada - 1997
Publicado de forma externa	Sí

Áreas temáticas de ASJC Scopus

Biofísica
Biología estructural
Bioquímica
Biología molecular
Genética
Biología celular

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title = "Expression and subcellular localization of mouse 20S proteasome activator complex PA28",

abstract = "We have cloned the mouse PA28 proteasome activator cDNAs. Northern blot demonstrates high PA28 mRNA levels in liver, kidney and lung, mRNA levels are low in thymus, spleen and brain. In contrast, PA28 protein levels vary little between these tissues. Immunocytological analysis and cell fractionation experiments demonstrate that both subunits are almost equally distributed between the cytoplasm and the nucleus. Interestingly, PA28α spares nucleoli, while PA28β is strongly enhanced in the nucleolus. This indicates for the first time that the PA28α and PA28β subunits may serve nuclear functions which may be different from and independent of each other.",

keywords = "Activator, Ki-autoantigen, Proteasome",

author = "Andrea Soza and Christine Knuehl and Marcus Groettrup and Peter Henklein and Keiji Tanaka and Kloetzel, {Peter M.}",

note = "Funding Information: We like to thank Dr. L. K{\"u}hn (D{\"u}sseldorf) for the generous gift of antibody hPA28 and purified human PA28. This work was supported by the Deutsche Forschungsgemeinschaft Grant Kl 427/9-1.",

year = "1997",

month = aug,

day = "11",

doi = "10.1016/S0014-5793(97)00864-8",

language = "English",

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journal = "FEBS Letters",

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T1 - Expression and subcellular localization of mouse 20S proteasome activator complex PA28

AU - Soza, Andrea

AU - Knuehl, Christine

AU - Groettrup, Marcus

AU - Henklein, Peter

AU - Tanaka, Keiji

AU - Kloetzel, Peter M.

N1 - Funding Information: We like to thank Dr. L. Kühn (Düsseldorf) for the generous gift of antibody hPA28 and purified human PA28. This work was supported by the Deutsche Forschungsgemeinschaft Grant Kl 427/9-1.

PY - 1997/8/11

Y1 - 1997/8/11

N2 - We have cloned the mouse PA28 proteasome activator cDNAs. Northern blot demonstrates high PA28 mRNA levels in liver, kidney and lung, mRNA levels are low in thymus, spleen and brain. In contrast, PA28 protein levels vary little between these tissues. Immunocytological analysis and cell fractionation experiments demonstrate that both subunits are almost equally distributed between the cytoplasm and the nucleus. Interestingly, PA28α spares nucleoli, while PA28β is strongly enhanced in the nucleolus. This indicates for the first time that the PA28α and PA28β subunits may serve nuclear functions which may be different from and independent of each other.

AB - We have cloned the mouse PA28 proteasome activator cDNAs. Northern blot demonstrates high PA28 mRNA levels in liver, kidney and lung, mRNA levels are low in thymus, spleen and brain. In contrast, PA28 protein levels vary little between these tissues. Immunocytological analysis and cell fractionation experiments demonstrate that both subunits are almost equally distributed between the cytoplasm and the nucleus. Interestingly, PA28α spares nucleoli, while PA28β is strongly enhanced in the nucleolus. This indicates for the first time that the PA28α and PA28β subunits may serve nuclear functions which may be different from and independent of each other.

KW - Activator

KW - Ki-autoantigen

KW - Proteasome

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U2 - 10.1016/S0014-5793(97)00864-8

DO - 10.1016/S0014-5793(97)00864-8

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AN - SCOPUS:0030840661

SN - 0014-5793

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SP - 27

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JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Expression and subcellular localization of mouse 20S proteasome activator complex PA28

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