TY - JOUR
T1 - Current technical approaches to brain energy metabolism
AU - Barros, L. Felipe
AU - Bolaños, Juan P.
AU - Bonvento, Gilles
AU - Bouzier-Sore, Anne Karine
AU - Brown, Angus
AU - Hirrlinger, Johannes
AU - Kasparov, Sergey
AU - Kirchhoff, Frank
AU - Murphy, Anne N.
AU - Pellerin, Luc
AU - Robinson, Michael B.
AU - Weber, Bruno
N1 - Publisher Copyright:
© 2017 Wiley Periodicals, Inc.
PY - 2018/6
Y1 - 2018/6
N2 - Neuroscience is a technology-driven discipline and brain energy metabolism is no exception. Once satisfied with mapping metabolic pathways at organ level, we are now looking to learn what it is exactly that metabolic enzymes and transporters do and when, where do they reside, how are they regulated, and how do they relate to the specific functions of neurons, glial cells, and their subcellular domains and organelles, in different areas of the brain. Moreover, we aim to quantify the fluxes of metabolites within and between cells. Energy metabolism is not just a necessity for proper cell function and viability but plays specific roles in higher brain functions such as memory processing and behavior, whose mechanisms need to be understood at all hierarchical levels, from isolated proteins to whole subjects, in both health and disease. To this aim, the field takes advantage of diverse disciplines including anatomy, histology, physiology, biochemistry, bioenergetics, cellular biology, molecular biology, developmental biology, neurology, and mathematical modeling. This article presents a well-referenced synopsis of the technical side of brain energy metabolism research. Detail and jargon are avoided whenever possible and emphasis is given to comparative strengths, limitations, and weaknesses, information that is often not available in regular articles.
AB - Neuroscience is a technology-driven discipline and brain energy metabolism is no exception. Once satisfied with mapping metabolic pathways at organ level, we are now looking to learn what it is exactly that metabolic enzymes and transporters do and when, where do they reside, how are they regulated, and how do they relate to the specific functions of neurons, glial cells, and their subcellular domains and organelles, in different areas of the brain. Moreover, we aim to quantify the fluxes of metabolites within and between cells. Energy metabolism is not just a necessity for proper cell function and viability but plays specific roles in higher brain functions such as memory processing and behavior, whose mechanisms need to be understood at all hierarchical levels, from isolated proteins to whole subjects, in both health and disease. To this aim, the field takes advantage of diverse disciplines including anatomy, histology, physiology, biochemistry, bioenergetics, cellular biology, molecular biology, developmental biology, neurology, and mathematical modeling. This article presents a well-referenced synopsis of the technical side of brain energy metabolism research. Detail and jargon are avoided whenever possible and emphasis is given to comparative strengths, limitations, and weaknesses, information that is often not available in regular articles.
KW - in vitro
KW - in vivo
KW - organization level
KW - spatio-temporal resolution
UR - http://www.scopus.com/inward/record.url?scp=85044365409&partnerID=8YFLogxK
U2 - 10.1002/glia.23248
DO - 10.1002/glia.23248
M3 - Review article
C2 - 29110344
AN - SCOPUS:85044365409
SN - 0894-1491
VL - 66
SP - 1138
EP - 1159
JO - GLIA
JF - GLIA
IS - 6
ER -