Comparative analysis of the stress and immune responses in Atlantic salmon (Salmo salar) inoculated with live and inactivated Piscirickettsia salmonis

Título traducido de la contribución: Analisis comparativo de la respuesta al estrés e inmune en salmón del Atlántico (Salmo salar) inoculado con Piscirickettsia salmonis viva e inactivada

Danixa Martínez*, Monserrat Garrido, Carlos Ponce, Yeraldine Zumelzu, Jose Coronado, Natacha Santibañez, Ana María Quilapi, Carolina Vargas-Lagos, Juan Pablo Pontigo, Ricardo Oyarzún-Salazar, Marcos Godoy, Ricardo Enríquez, Jose Luis Muñoz, Luis Vargas-Chacoff*, Alex Romero*

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

Resumen

Piscirickettsiosis causes the highest mortality in Atlantic salmon (Salmo salar) farming, and prophylactic treatment
has not provided complete protection to date. In this study, we analyzed the immune and metabolic responses
of Atlantic salmon inoculated with live and inactivated Piscirickettsia salmonis, monitoring plasma
markers related to immune and stress responses. The fish were inoculated with inactivated P. salmonis, live
P. salmonis, and culture medium (as control group). Blood and head-kidney samples were collected on days 3, 7,
and 14 post-inoculations (dpi). Glucose and lactate levels did not show statistical differences, while cortisol levels
increased from day 3 to day 14 in fish inoculated with live P. salmonis and only at 7 dpi in those inoculated with
inactivated P. salmonis. Furthermore, anti-P. salmonis IgM-type immunoglobulins increased up to 14 dpi in fish
inoculated with live P. salmonis but showed no change in those inoculated with inactivated P. salmonis. Meanwhile,
immune markers involved in type I responses (tnfα-1, ifnγ, and cd8β) and regulatory responses (il10, tgfβ-1,
and cd4-1) displayed differences between fish inoculated with live and inactivated P. salmonis. In fish inoculated
with live P. salmonis, there was a clear pattern of increase at both 3 and 14 dpi, while those inoculated with
inactivated P. salmonis showed a greater increase at 3 dpi. Our findings suggest that the nature of antigen may
influence humoral immunity (anti-P. salmonis IgM) and the gene expression of markers involved in type I and
regulatory immune responses in Atlantic salmon.
Título traducido de la contribuciónAnalisis comparativo de la respuesta al estrés e inmune en salmón del Atlántico (Salmo salar) inoculado con Piscirickettsia salmonis viva e inactivada
Idioma originalInglés
Número de artículo110111
PublicaciónFish and Shellfish Immunology
Volumen157
DOI
EstadoPublicada - 2025

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Áreas temáticas de ASJC Scopus

  • Inmunología
  • Química ambiental
  • Ciencias acuáticas
  • Inmunología y microbiología (miscelánea)

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