@article{b99ca1f6a4434170855370b5c4edb8d4,
title = "Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig ClC-5 chloride channel",
abstract = "We report the cloning of a guinea pig ClC-5 chloride channel (gpClC-5) from distal small intestinal epithelial cells by RT-PCR and RACE. The transcript is shown to be present in duodenum, jejunum and ileum epithelium by RT-PCR and Northern analysis. This is confirmed by in situ hybridisation which also shows the transcript to be homogeneously distributed in the crypt and villus regions. Expression of gpClC-5 in HEK-293 cells generated markedly outwardly rectified chloride currents with a perm-selectivity sequence of NO3- > Cl- > Br- > I- > F- > gluconate-. The possible role of gpClC-5 in this epithelial location is discussed.",
keywords = "Chloride channel, Epithelial transport, Guinea pig cloride channel protein-5, Patch clamp, Small intestine",
author = "Isabel Cornejo and Niemeyer, {Mar{\'i}a Isabel} and Sep{\'u}lveda, {Francisco V.} and Cid, {L. Pablo}",
note = "Funding Information: Supported by Grant 1990939 from Fondecyt (Chile) and the Volkswagen Stiftung (Germany). We are grateful to Prof. Thomas J. Jentsch for his kind gift of hClC-4 and 5 cDNAs. Institutional support to the Centro de Estudios Cient{\'ı}ficos (CECS) from a group of Chilean private companies (Codelco, Dimacofi, Empresas CMPC, Masisa S.A. and Telef{\'o}nica del Sur) is also acknowledged. The research of F.V.S. was supported in part by an International Research Scholars grant from the Howard Hughes Medical Institute. CECS is a Millennium Science Institute.",
year = "2001",
month = jun,
day = "6",
doi = "10.1016/S0005-2736(01)00331-5",
language = "English",
volume = "1512",
pages = "367--374",
journal = "Biochimica et Biophysica Acta - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier B.V.",
number = "2",
}