TY - JOUR
T1 - Catecholamine production along the nephron
AU - Di Marco, Giovana Seno
AU - Vio, Carlos P.
AU - Dos Santos, Oscar Fernando Pavão
AU - Schor, Nestor
AU - Casarini, Dulce Elena
PY - 2007
Y1 - 2007
N2 - The present work proposes an extra neural site of catecholamine production along the nephron. LLC-PK 1 , MDCK, and mIMCD-3 (proximal and distal tubules and inner medullary collecting duct, respectively) presented the following amine concentrations in the cell homogenates: Norepinephrine = 275±34, 56±16 and 255±21; Epinephrine = 161±20, 83±17 and 53±7; and Dopamine = 63±15, 39±6 and 36±7 pg/mg cell protein (Means ± SEM), respectively. The culture medium showed Norepinephrine = 168±25, 22±3 and 135±8; Epinephrine = 32±6, 152±17 and 39±5; and Dopamine = 27±9, 241±34 and 26±5 pg/mg cell protein, respectively. The synthesis enzymes as tyrosine hydroxylase, dopa decarboxylase and dopamine β-hydroxylase were detected by Western blotting. Biopterin, the enzymatic cofactor of tyrosine hydroxylase, was quantified in the intracellular and medium of mIMCD-3 cells (17±4 and 24±3 nmol/mg cell protein, respectively) and in the medium of MDCK cells (19±4 nmol/mg cell protein). The data confirmed that the proximal tubule is an important source of dopa decarboxilase and Dopamine and epithelial cell along the nephron express the biochemical pathway for catecholamine production.
AB - The present work proposes an extra neural site of catecholamine production along the nephron. LLC-PK 1 , MDCK, and mIMCD-3 (proximal and distal tubules and inner medullary collecting duct, respectively) presented the following amine concentrations in the cell homogenates: Norepinephrine = 275±34, 56±16 and 255±21; Epinephrine = 161±20, 83±17 and 53±7; and Dopamine = 63±15, 39±6 and 36±7 pg/mg cell protein (Means ± SEM), respectively. The culture medium showed Norepinephrine = 168±25, 22±3 and 135±8; Epinephrine = 32±6, 152±17 and 39±5; and Dopamine = 27±9, 241±34 and 26±5 pg/mg cell protein, respectively. The synthesis enzymes as tyrosine hydroxylase, dopa decarboxylase and dopamine β-hydroxylase were detected by Western blotting. Biopterin, the enzymatic cofactor of tyrosine hydroxylase, was quantified in the intracellular and medium of mIMCD-3 cells (17±4 and 24±3 nmol/mg cell protein, respectively) and in the medium of MDCK cells (19±4 nmol/mg cell protein). The data confirmed that the proximal tubule is an important source of dopa decarboxilase and Dopamine and epithelial cell along the nephron express the biochemical pathway for catecholamine production.
KW - Catecholamines
KW - Nephron
KW - Renal cells
KW - Tyrosine hydroxylase
UR - http://www.scopus.com/inward/record.url?scp=35949000516&partnerID=8YFLogxK
U2 - 10.1159/000110452
DO - 10.1159/000110452
M3 - Article
C2 - 17982274
AN - SCOPUS:35949000516
SN - 1015-8987
VL - 20
SP - 919
EP - 924
JO - Cellular Physiology and Biochemistry
JF - Cellular Physiology and Biochemistry
IS - 6
ER -