Analysis of tissue-resident immune cells from mouse skin and lungs by flow cytometry

Felipe Galvez-Cancino; Ernesto Lopez; Alvaro Lladser

doi:10.1007/978-1-4939-8979-9_16

Analysis of tissue-resident immune cells from mouse skin and lungs by flow cytometry

Felipe Galvez-Cancino, Ernesto Lopez, Alvaro Lladser^*

^*Autor correspondiente de este trabajo

Fundación Ciencia and Vida

Producción científica: Capítulo del libro/informe/acta de congreso › Capítulo › revisión exhaustiva

4 Citas (Scopus)

Resumen

The heterogeneity and complexity of nonlymphoid tissues has become a major obstacle for the study of immune populations. For this reason, the generation of highly reproducible protocols that allow the analysis of immune cells in these tissues has become crucial for clinical and preclinical research. Here we describe an optimized method that allows the obtention of single-cell suspensions from the skin and lungs to analyze and quantify populations of tissue-resident memory CD8 ⁺ T cells by multi-parametric flow cytometry.

Idioma original	Inglés
Título de la publicación alojada	Methods in Molecular Biology
Editorial	Humana Press Inc.
Páginas	217-222
Número de páginas	6
DOI	https://doi.org/10.1007/978-1-4939-8979-9_16
Estado	Publicada - 2019
Publicado de forma externa	Sí

Serie de la publicación

Nombre	Methods in Molecular Biology
Volumen	1913
ISSN (versión impresa)	1064-3745

Nota bibliográfica

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.

Áreas temáticas de ASJC Scopus

Biología molecular
Genética

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10.1007/978-1-4939-8979-9_16

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abstract = " The heterogeneity and complexity of nonlymphoid tissues has become a major obstacle for the study of immune populations. For this reason, the generation of highly reproducible protocols that allow the analysis of immune cells in these tissues has become crucial for clinical and preclinical research. Here we describe an optimized method that allows the obtention of single-cell suspensions from the skin and lungs to analyze and quantify populations of tissue-resident memory CD8 + T cells by multi-parametric flow cytometry.",

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AU - Lopez, Ernesto

AU - Lladser, Alvaro

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AB - The heterogeneity and complexity of nonlymphoid tissues has become a major obstacle for the study of immune populations. For this reason, the generation of highly reproducible protocols that allow the analysis of immune cells in these tissues has become crucial for clinical and preclinical research. Here we describe an optimized method that allows the obtention of single-cell suspensions from the skin and lungs to analyze and quantify populations of tissue-resident memory CD8 + T cells by multi-parametric flow cytometry.

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KW - Flow cytometry

KW - Immunophenotyping

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KW - Single-cell suspensions

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Analysis of tissue-resident immune cells from mouse skin and lungs by flow cytometry

Resumen

Serie de la publicación

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Áreas temáticas de ASJC Scopus

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