Tellurite reduction by Escherichia coli NDH-II dehydrogenase results in superoxide production in membranes of toxicant-exposed cells

Waldo A. Díaz-Vásquez; María J. Abarca-Lagunas; Felipe A. Arenas; Camilo A. Pinto; Fabián A. Cornejo; Poorna T. Wansapura; Gayan A. Appuhamillage; Thomas G. Chasteen; Claudio C. Vásquez

doi:10.1007/s10534-013-9701-8

Tellurite reduction by Escherichia coli NDH-II dehydrogenase results in superoxide production in membranes of toxicant-exposed cells

Waldo A. Díaz-Vásquez, María J. Abarca-Lagunas, Felipe A. Arenas, Camilo A. Pinto, Fabián A. Cornejo, Poorna T. Wansapura, Gayan A. Appuhamillage, Thomas G. Chasteen, Claudio C. Vásquez^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Tellurite, the most soluble tellurium oxyanion, is extremely harmful for most microorganisms. Part of this toxicity is due to the generation of reactive oxygen species that in turn cause oxidative stress. However, the way in which tellurite interferes with cellular processes is not well understood to date. Looking for new cellular tellurite targets, we decided to evaluate the functioning of the electron transport chain in tellurite-exposed cells. In this communication we show that the E. coli ndh gene, encoding NDH-II dehydrogenase, is significantly induced in toxicant-exposed cells and that the enzyme displays tellurite-reducing activity that results in increased superoxide levels in vitro.

Original language	English
Pages (from-to)	237-246
Number of pages	10
Journal	BioMetals
Volume	27
Issue number	2
DOIs	https://doi.org/10.1007/s10534-013-9701-8
State	Published - 2014

Bibliographical note

Funding Information:
Acknowledgments The authors thank Dr. J.A. Imlay for providing some of the microorganisms detailed in Table 1. This work received financial support from FONDECYT (Fondo Nacional de Ciencia y Tecnología) 1090097 and DICYT (Dirección de Investigación en Ciencia y Tecnología, Universidad de Santiago de Chile) (CCV). A doctoral fellowship and Grant No. 24121087 from CONICYT (Comisión Nacional de Ciencia y Tecnología) to WAD-V is also acknowledged. Support from the Robert A. Welch Foundation (X-011) is also gratefully acknowledged for the work at Sam Houston State University.

ASJC Scopus subject areas

Biomaterials
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
Metals and Alloys

Access to Document

10.1007/s10534-013-9701-8

Cite this

Díaz-Vásquez, W. A., Abarca-Lagunas, M. J., Arenas, F. A., Pinto, C. A., Cornejo, F. A., Wansapura, P. T., Appuhamillage, G. A., Chasteen, T. G., & Vásquez, C. C. (2014). Tellurite reduction by Escherichia coli NDH-II dehydrogenase results in superoxide production in membranes of toxicant-exposed cells. BioMetals, 27(2), 237-246. https://doi.org/10.1007/s10534-013-9701-8

@article{1c45490f11e34f9e98437076eea4784b,

title = "Tellurite reduction by Escherichia coli NDH-II dehydrogenase results in superoxide production in membranes of toxicant-exposed cells",

abstract = "Tellurite, the most soluble tellurium oxyanion, is extremely harmful for most microorganisms. Part of this toxicity is due to the generation of reactive oxygen species that in turn cause oxidative stress. However, the way in which tellurite interferes with cellular processes is not well understood to date. Looking for new cellular tellurite targets, we decided to evaluate the functioning of the electron transport chain in tellurite-exposed cells. In this communication we show that the E. coli ndh gene, encoding NDH-II dehydrogenase, is significantly induced in toxicant-exposed cells and that the enzyme displays tellurite-reducing activity that results in increased superoxide levels in vitro.",

keywords = "Electron transport chain, Escherichia coli, NDH-II, Superoxide, Tellurite, Tellurite reductase",

author = "D{\'i}az-V{\'a}squez, {Waldo A.} and Abarca-Lagunas, {Mar{\'i}a J.} and Arenas, {Felipe A.} and Pinto, {Camilo A.} and Cornejo, {Fabi{\'a}n A.} and Wansapura, {Poorna T.} and Appuhamillage, {Gayan A.} and Chasteen, {Thomas G.} and V{\'a}squez, {Claudio C.}",

note = "Funding Information: Acknowledgments The authors thank Dr. J.A. Imlay for providing some of the microorganisms detailed in Table 1. This work received financial support from FONDECYT (Fondo Nacional de Ciencia y Tecnolog{\'i}a) 1090097 and DICYT (Direcci{\'o}n de Investigaci{\'o}n en Ciencia y Tecnolog{\'i}a, Universidad de Santiago de Chile) (CCV). A doctoral fellowship and Grant No. 24121087 from CONICYT (Comisi{\'o}n Nacional de Ciencia y Tecnolog{\'i}a) to WAD-V is also acknowledged. Support from the Robert A. Welch Foundation (X-011) is also gratefully acknowledged for the work at Sam Houston State University.",

year = "2014",

month = apr,

doi = "10.1007/s10534-013-9701-8",

language = "English",

volume = "27",

pages = "237--246",

journal = "BioMetals",

issn = "0966-0844",

publisher = "Springer Science and Business Media B.V.",

number = "2",

}

TY - JOUR

T1 - Tellurite reduction by Escherichia coli NDH-II dehydrogenase results in superoxide production in membranes of toxicant-exposed cells

AU - Díaz-Vásquez, Waldo A.

AU - Abarca-Lagunas, María J.

AU - Arenas, Felipe A.

AU - Pinto, Camilo A.

AU - Cornejo, Fabián A.

AU - Wansapura, Poorna T.

AU - Appuhamillage, Gayan A.

AU - Chasteen, Thomas G.

AU - Vásquez, Claudio C.

N1 - Funding Information: Acknowledgments The authors thank Dr. J.A. Imlay for providing some of the microorganisms detailed in Table 1. This work received financial support from FONDECYT (Fondo Nacional de Ciencia y Tecnología) 1090097 and DICYT (Dirección de Investigación en Ciencia y Tecnología, Universidad de Santiago de Chile) (CCV). A doctoral fellowship and Grant No. 24121087 from CONICYT (Comisión Nacional de Ciencia y Tecnología) to WAD-V is also acknowledged. Support from the Robert A. Welch Foundation (X-011) is also gratefully acknowledged for the work at Sam Houston State University.

PY - 2014/4

Y1 - 2014/4

N2 - Tellurite, the most soluble tellurium oxyanion, is extremely harmful for most microorganisms. Part of this toxicity is due to the generation of reactive oxygen species that in turn cause oxidative stress. However, the way in which tellurite interferes with cellular processes is not well understood to date. Looking for new cellular tellurite targets, we decided to evaluate the functioning of the electron transport chain in tellurite-exposed cells. In this communication we show that the E. coli ndh gene, encoding NDH-II dehydrogenase, is significantly induced in toxicant-exposed cells and that the enzyme displays tellurite-reducing activity that results in increased superoxide levels in vitro.

AB - Tellurite, the most soluble tellurium oxyanion, is extremely harmful for most microorganisms. Part of this toxicity is due to the generation of reactive oxygen species that in turn cause oxidative stress. However, the way in which tellurite interferes with cellular processes is not well understood to date. Looking for new cellular tellurite targets, we decided to evaluate the functioning of the electron transport chain in tellurite-exposed cells. In this communication we show that the E. coli ndh gene, encoding NDH-II dehydrogenase, is significantly induced in toxicant-exposed cells and that the enzyme displays tellurite-reducing activity that results in increased superoxide levels in vitro.

KW - Electron transport chain

KW - Escherichia coli

KW - NDH-II

KW - Superoxide

KW - Tellurite

KW - Tellurite reductase

UR - http://www.scopus.com/inward/record.url?scp=84896319336&partnerID=8YFLogxK

U2 - 10.1007/s10534-013-9701-8

DO - 10.1007/s10534-013-9701-8

M3 - Article

C2 - 24481550

AN - SCOPUS:84896319336

SN - 0966-0844

VL - 27

SP - 237

EP - 246

JO - BioMetals

JF - BioMetals

IS - 2

ER -

Tellurite reduction by Escherichia coli NDH-II dehydrogenase results in superoxide production in membranes of toxicant-exposed cells

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this