T-cell homing to the gut mucosa: General concepts and methodological considerations

Jaime De Calisto, Eduardo J. Villablanca, Sen Wang, Maria R. Bono, Mario Rosemblatt, J. Rodrigo Mora*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

23 Scopus citations

Abstract

Effector/memory T cells can migrate to most extra-lymphoid tissues in the body. However, migration to the intestinal mucosa requires the expression of very specific homing receptors on T cells, integrin α4β7 and chemokine receptor CCR9. These receptors are induced by all-trans retinoic acid (RA), a vitamin A metabolite that is specifically synthesized by gut-associated dendritic cells (DC), but not by extra-intestinal DC. Here we summarize some general concepts on T cell homing with an emphasis on the gut mucosa. We also discuss experimental strategies to generate gut-homing T cells in vivo and in vitro and the techniques to track gut-homing T cells.

Original languageEnglish
Title of host publicationIntegrin and Cell Adhesion Molecules
Subtitle of host publicationMethods and Protocols
EditorsMotomu Shimaoka, Motomu Shimaoka
Pages411-434
Number of pages24
DOIs
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume757
ISSN (Print)1064-3745

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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