Synthesis and expression of functional angiotensin II receptors in Xenopus oocytes injected with rat brain mRNA

Daniel Cross; Freddy Cifuentes; J. Pablo Huidobro-Toro; Carlos P. Vío; Nibaldo C. Inestrosa

doi:10.1016/0169-328X(87)90034-9

Synthesis and expression of functional angiotensin II receptors in Xenopus oocytes injected with rat brain mRNA

Daniel Cross, Freddy Cifuentes, J. Pablo Huidobro-Toro, Carlos P. Vío, Nibaldo C. Inestrosa^*

^*Corresponding author for this work

Pontificia Universidad Católica de Chile

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Xenopus laevis oocytes were injected with poly(A)⁺ mRNA isolated from rat brain and superfused in a medium containing either serotonin, angiotensin II or bradykinin. Applications of serotonin or angiotensin II to injected oocytes elicited, in a dose-dependent manner, changes in membrane potential. The angiotensin II receptor was desensitized fairly rapidly in the continued presence of the agonist. No response was obtained with bradykinin. The selectivity of the angiotensin II-induced response was demonstrated by the finding that the angiotensin II antagonist ([Sar¹,Ala⁸]angiotensin II, saralasin) blocked the angiotensin II-induced response. It is concluded that an appropriate fraction of brain mRNA is capable of directing the synthesis and correct insertion of functional angiotensin II receptors in the Xenopus oocyte membrane.

Original language	English
Pages (from-to)	268-270
Number of pages	3
Journal	Molecular Brain Research
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/0169-328X(87)90034-9
State	Published - 1987
Externally published	Yes

Bibliographical note

Funding Information:
We would like to thank Drs. C. Allende and P. Carvallo for introducing us to the technique of the oocyte injection. We are also indebted to Dr. J. Szec6wka for helpful advice. This study was supported in part by Grants from the Stiftung Volkswa-genwerk, the Research Direction of the Catholic University and from the Gildemeister Foundation to N. C.I. and Dr. Jaime Alvarez.

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/0169-328X(87)90034-9

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title = "Synthesis and expression of functional angiotensin II receptors in Xenopus oocytes injected with rat brain mRNA",

abstract = "Xenopus laevis oocytes were injected with poly(A)+ mRNA isolated from rat brain and superfused in a medium containing either serotonin, angiotensin II or bradykinin. Applications of serotonin or angiotensin II to injected oocytes elicited, in a dose-dependent manner, changes in membrane potential. The angiotensin II receptor was desensitized fairly rapidly in the continued presence of the agonist. No response was obtained with bradykinin. The selectivity of the angiotensin II-induced response was demonstrated by the finding that the angiotensin II antagonist ([Sar1,Ala8]angiotensin II, saralasin) blocked the angiotensin II-induced response. It is concluded that an appropriate fraction of brain mRNA is capable of directing the synthesis and correct insertion of functional angiotensin II receptors in the Xenopus oocyte membrane.",

keywords = "Angiotensin II receptor, Brain mRNA, Microinjection, Saralasin, Xenopus laevis oocyte",

author = "Daniel Cross and Freddy Cifuentes and Huidobro-Toro, {J. Pablo} and V{\'i}o, {Carlos P.} and Inestrosa, {Nibaldo C.}",

note = "Funding Information: We would like to thank Drs. C. Allende and P. Carvallo for introducing us to the technique of the oocyte injection. We are also indebted to Dr. J. Szec6wka for helpful advice. This study was supported in part by Grants from the Stiftung Volkswa-genwerk, the Research Direction of the Catholic University and from the Gildemeister Foundation to N. C.I. and Dr. Jaime Alvarez.",

year = "1987",

month = sep,

doi = "10.1016/0169-328X(87)90034-9",

language = "English",

volume = "2",

pages = "268--270",

journal = "Molecular Brain Research",

issn = "0169-328X",

publisher = "Elsevier B.V.",

number = "3",

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TY - JOUR

T1 - Synthesis and expression of functional angiotensin II receptors in Xenopus oocytes injected with rat brain mRNA

AU - Cross, Daniel

AU - Cifuentes, Freddy

AU - Huidobro-Toro, J. Pablo

AU - Vío, Carlos P.

AU - Inestrosa, Nibaldo C.

N1 - Funding Information: We would like to thank Drs. C. Allende and P. Carvallo for introducing us to the technique of the oocyte injection. We are also indebted to Dr. J. Szec6wka for helpful advice. This study was supported in part by Grants from the Stiftung Volkswa-genwerk, the Research Direction of the Catholic University and from the Gildemeister Foundation to N. C.I. and Dr. Jaime Alvarez.

PY - 1987/9

Y1 - 1987/9

N2 - Xenopus laevis oocytes were injected with poly(A)+ mRNA isolated from rat brain and superfused in a medium containing either serotonin, angiotensin II or bradykinin. Applications of serotonin or angiotensin II to injected oocytes elicited, in a dose-dependent manner, changes in membrane potential. The angiotensin II receptor was desensitized fairly rapidly in the continued presence of the agonist. No response was obtained with bradykinin. The selectivity of the angiotensin II-induced response was demonstrated by the finding that the angiotensin II antagonist ([Sar1,Ala8]angiotensin II, saralasin) blocked the angiotensin II-induced response. It is concluded that an appropriate fraction of brain mRNA is capable of directing the synthesis and correct insertion of functional angiotensin II receptors in the Xenopus oocyte membrane.

AB - Xenopus laevis oocytes were injected with poly(A)+ mRNA isolated from rat brain and superfused in a medium containing either serotonin, angiotensin II or bradykinin. Applications of serotonin or angiotensin II to injected oocytes elicited, in a dose-dependent manner, changes in membrane potential. The angiotensin II receptor was desensitized fairly rapidly in the continued presence of the agonist. No response was obtained with bradykinin. The selectivity of the angiotensin II-induced response was demonstrated by the finding that the angiotensin II antagonist ([Sar1,Ala8]angiotensin II, saralasin) blocked the angiotensin II-induced response. It is concluded that an appropriate fraction of brain mRNA is capable of directing the synthesis and correct insertion of functional angiotensin II receptors in the Xenopus oocyte membrane.

KW - Angiotensin II receptor

KW - Brain mRNA

KW - Microinjection

KW - Saralasin

KW - Xenopus laevis oocyte

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U2 - 10.1016/0169-328X(87)90034-9

DO - 10.1016/0169-328X(87)90034-9

M3 - Article

C2 - 3676711

AN - SCOPUS:0023247146

SN - 0169-328X

VL - 2

SP - 268

EP - 270

JO - Molecular Brain Research

JF - Molecular Brain Research

IS - 3

ER -

Synthesis and expression of functional angiotensin II receptors in Xenopus oocytes injected with rat brain mRNA

Abstract

Bibliographical note

ASJC Scopus subject areas

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