PA28αβ double and PA28β single transfectant mouse B8 cell lines reveal enhanced presentation of a mouse cytomegalovirus (MCMV) pp89 MHC class I epitope

Ralf Stohwasser; Andrea Soza; Maren Eggers; Ulrich H. Koszinowski; Peter M. Kloetzel

doi:10.1016/S0161-5890(00)00017-1

PA28αβ double and PA28β single transfectant mouse B8 cell lines reveal enhanced presentation of a mouse cytomegalovirus (MCMV) pp89 MHC class I epitope

Ralf Stohwasser^*, Andrea Soza, Maren Eggers, Ulrich H. Koszinowski, Peter M. Kloetzel

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

PA28 is an interferon-γ inducible modulator of proteasome function composed of two subunits, i.e. PA28α and PA28β. Previously we showed that stabile overexpression of the PA28α subunit alone supported MHC class I antigen presentation of two viral epitopes. However, no information was obtained on the consequences when PA28α and PA28β function in concert or when PA28β is overexpressed on its own. Here we demonstrate that overexpression of PA28α and β together is similarly efficient in supporting MHC class I antigen presentation of the MCMV pp89 9mer epitope as PA28α alone, excluding a potentially potentiating role of PA28β. Surprisingly, and despite the fact that PA28β alone was thought to be inactive and to only stabilize PA28 activity, overexpression of PA28β also resulted in improved antigen presentation. However, by northernblot and immunoprecipitation we show that while PA28α is able to act alone the observed effect in the PA28β and PA28αβ transfectant cell lines is due to increased levels of PA28αβ complexes. (C) 2000 Elsevier Science Ltd. All rights reserved.

Original language	English
Pages (from-to)	13-19
Number of pages	7
Journal	Molecular Immunology
Volume	37
Issue number	1-2
DOIs	https://doi.org/10.1016/S0161-5890(00)00017-1
State	Published - 2000
Externally published	Yes

Bibliographical note

Funding Information:
We would like to thank Hartmut Hengel for antibodies applied to study pp89 expression (rabbit serum b5/1; mAb 6-58.1). We would like to thank Dr M. Groettrup, Ulrike Salzmann and Jan Giesebrecht for support in some of the experiments, Dr A. Sijts for helpful discussions and S. Standera and I. Peters for technical assistance. The work was supported by grants of the Deutsche Forschungsgemeinschaft (Kl 427/ 9-2; 10-1) and EU: Bio4-CT-0505 to Dr Kloetzel and Dr Koszinowski.

ASJC Scopus subject areas

Immunology
Molecular Biology

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10.1016/S0161-5890(00)00017-1

Cite this

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title = "PA28αβ double and PA28β single transfectant mouse B8 cell lines reveal enhanced presentation of a mouse cytomegalovirus (MCMV) pp89 MHC class I epitope",

abstract = "PA28 is an interferon-γ inducible modulator of proteasome function composed of two subunits, i.e. PA28α and PA28β. Previously we showed that stabile overexpression of the PA28α subunit alone supported MHC class I antigen presentation of two viral epitopes. However, no information was obtained on the consequences when PA28α and PA28β function in concert or when PA28β is overexpressed on its own. Here we demonstrate that overexpression of PA28α and β together is similarly efficient in supporting MHC class I antigen presentation of the MCMV pp89 9mer epitope as PA28α alone, excluding a potentially potentiating role of PA28β. Surprisingly, and despite the fact that PA28β alone was thought to be inactive and to only stabilize PA28 activity, overexpression of PA28β also resulted in improved antigen presentation. However, by northernblot and immunoprecipitation we show that while PA28α is able to act alone the observed effect in the PA28β and PA28αβ transfectant cell lines is due to increased levels of PA28αβ complexes. (C) 2000 Elsevier Science Ltd. All rights reserved.",

keywords = "MHC class I antigen processing, Murine cytomegalovirus, PA28αβ, Proteasome",

author = "Ralf Stohwasser and Andrea Soza and Maren Eggers and Koszinowski, {Ulrich H.} and Kloetzel, {Peter M.}",

note = "Funding Information: We would like to thank Hartmut Hengel for antibodies applied to study pp89 expression (rabbit serum b5/1; mAb 6-58.1). We would like to thank Dr M. Groettrup, Ulrike Salzmann and Jan Giesebrecht for support in some of the experiments, Dr A. Sijts for helpful discussions and S. Standera and I. Peters for technical assistance. The work was supported by grants of the Deutsche Forschungsgemeinschaft (Kl 427/ 9-2; 10-1) and EU: Bio4-CT-0505 to Dr Kloetzel and Dr Koszinowski.",

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AU - Stohwasser, Ralf

AU - Soza, Andrea

AU - Eggers, Maren

AU - Koszinowski, Ulrich H.

AU - Kloetzel, Peter M.

N1 - Funding Information: We would like to thank Hartmut Hengel for antibodies applied to study pp89 expression (rabbit serum b5/1; mAb 6-58.1). We would like to thank Dr M. Groettrup, Ulrike Salzmann and Jan Giesebrecht for support in some of the experiments, Dr A. Sijts for helpful discussions and S. Standera and I. Peters for technical assistance. The work was supported by grants of the Deutsche Forschungsgemeinschaft (Kl 427/ 9-2; 10-1) and EU: Bio4-CT-0505 to Dr Kloetzel and Dr Koszinowski.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - PA28 is an interferon-γ inducible modulator of proteasome function composed of two subunits, i.e. PA28α and PA28β. Previously we showed that stabile overexpression of the PA28α subunit alone supported MHC class I antigen presentation of two viral epitopes. However, no information was obtained on the consequences when PA28α and PA28β function in concert or when PA28β is overexpressed on its own. Here we demonstrate that overexpression of PA28α and β together is similarly efficient in supporting MHC class I antigen presentation of the MCMV pp89 9mer epitope as PA28α alone, excluding a potentially potentiating role of PA28β. Surprisingly, and despite the fact that PA28β alone was thought to be inactive and to only stabilize PA28 activity, overexpression of PA28β also resulted in improved antigen presentation. However, by northernblot and immunoprecipitation we show that while PA28α is able to act alone the observed effect in the PA28β and PA28αβ transfectant cell lines is due to increased levels of PA28αβ complexes. (C) 2000 Elsevier Science Ltd. All rights reserved.

AB - PA28 is an interferon-γ inducible modulator of proteasome function composed of two subunits, i.e. PA28α and PA28β. Previously we showed that stabile overexpression of the PA28α subunit alone supported MHC class I antigen presentation of two viral epitopes. However, no information was obtained on the consequences when PA28α and PA28β function in concert or when PA28β is overexpressed on its own. Here we demonstrate that overexpression of PA28α and β together is similarly efficient in supporting MHC class I antigen presentation of the MCMV pp89 9mer epitope as PA28α alone, excluding a potentially potentiating role of PA28β. Surprisingly, and despite the fact that PA28β alone was thought to be inactive and to only stabilize PA28 activity, overexpression of PA28β also resulted in improved antigen presentation. However, by northernblot and immunoprecipitation we show that while PA28α is able to act alone the observed effect in the PA28β and PA28αβ transfectant cell lines is due to increased levels of PA28αβ complexes. (C) 2000 Elsevier Science Ltd. All rights reserved.

KW - MHC class I antigen processing

KW - Murine cytomegalovirus

KW - PA28αβ

KW - Proteasome

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SN - 0161-5890

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ER -

PA28αβ double and PA28β single transfectant mouse B8 cell lines reveal enhanced presentation of a mouse cytomegalovirus (MCMV) pp89 MHC class I epitope

Abstract

Bibliographical note

ASJC Scopus subject areas

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