Novel Microsynthesis of High-Yield Gold Nanoparticles to Accelerate Research in Biosensing and Other Bioapplications

Víctor Manuel Díaz García*, Astrid Haensgen, Braulio Contreras Trigo, Patricio Alejandro Oyarzún Cayo*, Maria Ligia Ligia Inostroza Concha

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Gold nanoparticles (AuNPs) exhibit unique properties that make them appealing for applications in biosensing and other emerging fields. Despite the availability of numerous synthesis methods, important questions remain to be addressed regarding the volume effect on the synthesis yield and quality of AuNPs in the light of biosensing research. The present study addresses these issues by developing a novel microvolumetric citrate-reduction method to improve the synthesis of AuNPs, which were characterized by electronic microscopy, energy dispersive spectroscopy, zeta potential and colorimetric analysis. A comparison of the novel microsynthesis method with the standard Turkevich method demonstrated its superior performance in terms of yield, monodispersity, rapidity (in one step), reproducibility, and stability. The analytical behavior of AuNPs-based aptasensors prepared by microsynthesis was investigated using kanamycin detection and showed higher reproducibility and improved detection limits (3.4 times) compared to those of Turkevich AuNPs. Finally, the effect of pH was studied to demonstrate the suitability of the method for the screening of AuNP synthesis parameters that are of direct interest in biosensing research; the results showed an optimal pH range between 5.0 and 5.5. In summary, the approach described herein has the potential to improve research capabilities in biosensing, with the added benefits of lowering costs and minimizing waste generation in line with current trends in green nanotechnology.

Original languageEnglish
Article number992
Pages (from-to)1-18
Number of pages18
Issue number12
StatePublished - 2023

Bibliographical note

Publisher Copyright:
© 2023 by the authors.

ASJC Scopus subject areas

  • General Chemistry
  • General Engineering


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