MUC1/SEC and MUC1/Y overexpression is associated with inflammation in Sjögren's syndrome

H. H. Sung; I. Castro; S. González; S. Aguilera; N. I. Smorodinsky; A. F.G. Quest; V. Bahamondes; C. Alliende; J. Cortés; C. Molina; U. Urzúa; M. J. Barrera; M. Hermoso; L. Herrera; C. Leyton; M. J. González

doi:10.1111/odi.12339

MUC1/SEC and MUC1/Y overexpression is associated with inflammation in Sjögren's syndrome

H. H. Sung, I. Castro, S. González, S. Aguilera, N. I. Smorodinsky, A. F.G. Quest, V. Bahamondes, C. Alliende, J. Cortés, C. Molina, U. Urzúa, M. J. Barrera, M. Hermoso, L. Herrera, C. Leyton, M. J. González^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Objectives: To evaluate the expression and localization of MUC1/SEC and MUC1/Y isoforms in labial salivary glands (LSG) from Sjögren's syndrome patients (SS patients), as well as their in vitro expression induced by cytokines. Subjects and methods: Labial salivary gland from 27 primary SS patients and 22 non-SS sicca subjects were studied. Relative MUC1/SEC and MUC1/Y mRNA levels were determined by qPCR and protein levels by Western blotting. Induction of mucin mRNAs was assayed in vitro. Immunohistochemistry was used for localization. Results: Relative MUC1/SEC and MUC1/Y mRNA and protein levels were significantly higher in LSG from SS patients. These mRNAs were induced by cytokines. MUC1/SEC and MUC1/Y were detected in acini apical region of control LSGs, and significant cytoplasmic accumulation was observed in acini of SS patients. MUC1/Y localized in acinar nuclei and cytoplasm of inflammatory cells of LSG from SS patients. A strong positive correlation was observed between cellular MUC1/SEC levels and glandular function determined by scintigraphy. Conclusions: We show for the first time that MUC1/SEC and MUC1/Y are expressed in LSG of both SS patients and non-SS sicca subjects. The observed overexpression and aberrant localization of MUC1/SEC and MUC1/Y and their induction by pro-inflammatory cytokines may favor the perpetuation of the inflammatory environment that disrupts the salivary glandular homeostasis in SS patients.

Original language	English
Pages (from-to)	730-738
Number of pages	9
Journal	Oral Diseases
Volume	21
Issue number	6
DOIs	https://doi.org/10.1111/odi.12339
State	Published - 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 John Wiley & Sons A/S.

ASJC Scopus subject areas

Otorhinolaryngology
General Dentistry

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10.1111/odi.12339

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Sung, H. H., Castro, I., González, S., Aguilera, S., Smorodinsky, N. I., Quest, A. F. G., Bahamondes, V., Alliende, C., Cortés, J., Molina, C., Urzúa, U., Barrera, M. J., Hermoso, M., Herrera, L., Leyton, C., & González, M. J. (2015). MUC1/SEC and MUC1/Y overexpression is associated with inflammation in Sjögren's syndrome. Oral Diseases, 21(6), 730-738. https://doi.org/10.1111/odi.12339

@article{576eeaebdb4e425880b155cc82208a92,

title = "MUC1/SEC and MUC1/Y overexpression is associated with inflammation in Sj{\"o}gren's syndrome",

abstract = "Objectives: To evaluate the expression and localization of MUC1/SEC and MUC1/Y isoforms in labial salivary glands (LSG) from Sj{\"o}gren's syndrome patients (SS patients), as well as their in vitro expression induced by cytokines. Subjects and methods: Labial salivary gland from 27 primary SS patients and 22 non-SS sicca subjects were studied. Relative MUC1/SEC and MUC1/Y mRNA levels were determined by qPCR and protein levels by Western blotting. Induction of mucin mRNAs was assayed in vitro. Immunohistochemistry was used for localization. Results: Relative MUC1/SEC and MUC1/Y mRNA and protein levels were significantly higher in LSG from SS patients. These mRNAs were induced by cytokines. MUC1/SEC and MUC1/Y were detected in acini apical region of control LSGs, and significant cytoplasmic accumulation was observed in acini of SS patients. MUC1/Y localized in acinar nuclei and cytoplasm of inflammatory cells of LSG from SS patients. A strong positive correlation was observed between cellular MUC1/SEC levels and glandular function determined by scintigraphy. Conclusions: We show for the first time that MUC1/SEC and MUC1/Y are expressed in LSG of both SS patients and non-SS sicca subjects. The observed overexpression and aberrant localization of MUC1/SEC and MUC1/Y and their induction by pro-inflammatory cytokines may favor the perpetuation of the inflammatory environment that disrupts the salivary glandular homeostasis in SS patients.",

keywords = "Autoimmunity, Immuno-enhancer, Inflammation, MUC1/SEC, MUC1/Y, Salivary glands",

author = "Sung, {H. H.} and I. Castro and S. Gonz{\'a}lez and S. Aguilera and Smorodinsky, {N. I.} and Quest, {A. F.G.} and V. Bahamondes and C. Alliende and J. Cort{\'e}s and C. Molina and U. Urz{\'u}a and Barrera, {M. J.} and M. Hermoso and L. Herrera and C. Leyton and Gonz{\'a}lez, {M. J.}",

note = "Publisher Copyright: {\textcopyright} 2015 John Wiley & Sons A/S.",

year = "2015",

month = sep,

day = "1",

doi = "10.1111/odi.12339",

language = "English",

volume = "21",

pages = "730--738",

journal = "Oral Diseases",

issn = "1354-523X",

publisher = "Wiley-Blackwell",

number = "6",

}

Sung, HH, Castro, I, González, S, Aguilera, S, Smorodinsky, NI, Quest, AFG, Bahamondes, V, Alliende, C, Cortés, J, Molina, C, Urzúa, U, Barrera, MJ, Hermoso, M, Herrera, L, Leyton, C & González, MJ 2015, 'MUC1/SEC and MUC1/Y overexpression is associated with inflammation in Sjögren's syndrome', Oral Diseases, vol. 21, no. 6, pp. 730-738. https://doi.org/10.1111/odi.12339

TY - JOUR

T1 - MUC1/SEC and MUC1/Y overexpression is associated with inflammation in Sjögren's syndrome

AU - Sung, H. H.

AU - Castro, I.

AU - González, S.

AU - Aguilera, S.

AU - Smorodinsky, N. I.

AU - Quest, A. F.G.

AU - Bahamondes, V.

AU - Alliende, C.

AU - Cortés, J.

AU - Molina, C.

AU - Urzúa, U.

AU - Barrera, M. J.

AU - Hermoso, M.

AU - Herrera, L.

AU - Leyton, C.

AU - González, M. J.

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Objectives: To evaluate the expression and localization of MUC1/SEC and MUC1/Y isoforms in labial salivary glands (LSG) from Sjögren's syndrome patients (SS patients), as well as their in vitro expression induced by cytokines. Subjects and methods: Labial salivary gland from 27 primary SS patients and 22 non-SS sicca subjects were studied. Relative MUC1/SEC and MUC1/Y mRNA levels were determined by qPCR and protein levels by Western blotting. Induction of mucin mRNAs was assayed in vitro. Immunohistochemistry was used for localization. Results: Relative MUC1/SEC and MUC1/Y mRNA and protein levels were significantly higher in LSG from SS patients. These mRNAs were induced by cytokines. MUC1/SEC and MUC1/Y were detected in acini apical region of control LSGs, and significant cytoplasmic accumulation was observed in acini of SS patients. MUC1/Y localized in acinar nuclei and cytoplasm of inflammatory cells of LSG from SS patients. A strong positive correlation was observed between cellular MUC1/SEC levels and glandular function determined by scintigraphy. Conclusions: We show for the first time that MUC1/SEC and MUC1/Y are expressed in LSG of both SS patients and non-SS sicca subjects. The observed overexpression and aberrant localization of MUC1/SEC and MUC1/Y and their induction by pro-inflammatory cytokines may favor the perpetuation of the inflammatory environment that disrupts the salivary glandular homeostasis in SS patients.

AB - Objectives: To evaluate the expression and localization of MUC1/SEC and MUC1/Y isoforms in labial salivary glands (LSG) from Sjögren's syndrome patients (SS patients), as well as their in vitro expression induced by cytokines. Subjects and methods: Labial salivary gland from 27 primary SS patients and 22 non-SS sicca subjects were studied. Relative MUC1/SEC and MUC1/Y mRNA levels were determined by qPCR and protein levels by Western blotting. Induction of mucin mRNAs was assayed in vitro. Immunohistochemistry was used for localization. Results: Relative MUC1/SEC and MUC1/Y mRNA and protein levels were significantly higher in LSG from SS patients. These mRNAs were induced by cytokines. MUC1/SEC and MUC1/Y were detected in acini apical region of control LSGs, and significant cytoplasmic accumulation was observed in acini of SS patients. MUC1/Y localized in acinar nuclei and cytoplasm of inflammatory cells of LSG from SS patients. A strong positive correlation was observed between cellular MUC1/SEC levels and glandular function determined by scintigraphy. Conclusions: We show for the first time that MUC1/SEC and MUC1/Y are expressed in LSG of both SS patients and non-SS sicca subjects. The observed overexpression and aberrant localization of MUC1/SEC and MUC1/Y and their induction by pro-inflammatory cytokines may favor the perpetuation of the inflammatory environment that disrupts the salivary glandular homeostasis in SS patients.

KW - Autoimmunity

KW - Immuno-enhancer

KW - Inflammation

KW - MUC1/SEC

KW - MUC1/Y

KW - Salivary glands

UR - http://www.scopus.com/inward/record.url?scp=84938741260&partnerID=8YFLogxK

U2 - 10.1111/odi.12339

DO - 10.1111/odi.12339

M3 - Article

C2 - 25757505

AN - SCOPUS:84938741260

SN - 1354-523X

VL - 21

SP - 730

EP - 738

JO - Oral Diseases

JF - Oral Diseases

IS - 6

ER -

MUC1/SEC and MUC1/Y overexpression is associated with inflammation in Sjögren's syndrome

Abstract

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