Kidney-resident innate-like memory γδ T cells control chronic Staphylococcus aureus infection of mice

Tabea Bertram; Daniel Reimers; Niels C. Lory; Constantin Schmidt; Joanna Schmid; Lisa C. Heinig; Peter Bradtke; Guido Rattay; Stephanie Zielinski; Malte Hellmig; Patricia Bartsch; Holger Rohde; Sarah Nuñez; Mariana V. Rosemblatt; Maria Rosa Bono; Nicola Gagliani; Inga Sandrock; Ulf Panzer; Christian F. Krebs; Catherine Meyer-Schwesinger; Immo Prinz; Hans Willi Mittrücker

doi:10.1073/pnas.2210490120

Kidney-resident innate-like memory γδ T cells control chronic Staphylococcus aureus infection of mice

Tabea Bertram, Daniel Reimers, Niels C. Lory, Constantin Schmidt, Joanna Schmid, Lisa C. Heinig, Peter Bradtke, Guido Rattay, Stephanie Zielinski, Malte Hellmig, Patricia Bartsch, Holger Rohde, Sarah Nuñez, Mariana V. Rosemblatt, Maria Rosa Bono, Nicola Gagliani, Inga Sandrock, Ulf Panzer, Christian F. Krebs, Catherine Meyer-SchwesingerImmo Prinz, Hans Willi Mittrücker^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

γδ T cells are involved in the control of Staphylococcus aureus infection, but their importance in protection compared to other T cells is unclear. We used a mouse model of systemic S. aureus infection associated with high bacterial load and persistence in the kidney. Infection caused fulminant accumulation of γδ T cells in the kidney. Renal γδ T cells acquired tissue residency and were maintained in high numbers during chronic infection. At day 7, up to 50% of renal γδ T cells produced IL-17A in situ and a large fraction of renal γδ T cells remained IL-17A+ during chronic infection. Controlled depletion revealed that γδ T cells restricted renal S. aureus replication in the acute infection and provided protection during chronic renal infection and upon reinfection. Our results demonstrate that kidney-resident γδ T cells are nonredundant in limiting local S. aureus growth during chronic infection and provide enhanced protection against reinfection.

Original language	English
Article number	e2210490120
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	120
Issue number	1
DOIs	https://doi.org/10.1073/pnas.2210490120
State	Published - 2023

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Publisher Copyright:
© 2022 the Author(s).

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Bertram, T., Reimers, D., Lory, N. C., Schmidt, C., Schmid, J., Heinig, L. C., Bradtke, P., Rattay, G., Zielinski, S., Hellmig, M., Bartsch, P., Rohde, H., Nuñez, S., Rosemblatt, M. V., Rosa Bono, M., Gagliani, N., Sandrock, I., Panzer, U., Krebs, C. F., ... Mittrücker, H. W. (2023). Kidney-resident innate-like memory γδ T cells control chronic Staphylococcus aureus infection of mice. Proceedings of the National Academy of Sciences of the United States of America, 120(1), Article e2210490120. https://doi.org/10.1073/pnas.2210490120

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title = "Kidney-resident innate-like memory γδ T cells control chronic Staphylococcus aureus infection of mice",

abstract = "γδ T cells are involved in the control of Staphylococcus aureus infection, but their importance in protection compared to other T cells is unclear. We used a mouse model of systemic S. aureus infection associated with high bacterial load and persistence in the kidney. Infection caused fulminant accumulation of γδ T cells in the kidney. Renal γδ T cells acquired tissue residency and were maintained in high numbers during chronic infection. At day 7, up to 50% of renal γδ T cells produced IL-17A in situ and a large fraction of renal γδ T cells remained IL-17A+ during chronic infection. Controlled depletion revealed that γδ T cells restricted renal S. aureus replication in the acute infection and provided protection during chronic renal infection and upon reinfection. Our results demonstrate that kidney-resident γδ T cells are nonredundant in limiting local S. aureus growth during chronic infection and provide enhanced protection against reinfection.",

keywords = "IL-17, Staphylococcus aureus, T cell memory, tissue residency, γδ T cells",

author = "Tabea Bertram and Daniel Reimers and Lory, {Niels C.} and Constantin Schmidt and Joanna Schmid and Heinig, {Lisa C.} and Peter Bradtke and Guido Rattay and Stephanie Zielinski and Malte Hellmig and Patricia Bartsch and Holger Rohde and Sarah Nu{\~n}ez and Rosemblatt, {Mariana V.} and {Rosa Bono}, Maria and Nicola Gagliani and Inga Sandrock and Ulf Panzer and Krebs, {Christian F.} and Catherine Meyer-Schwesinger and Immo Prinz and Mittr{\"u}cker, {Hans Willi}",

note = "Publisher Copyright: {\textcopyright} 2022 the Author(s).",

year = "2023",

month = jan,

day = "3",

doi = "10.1073/pnas.2210490120",

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Bertram, T, Reimers, D, Lory, NC, Schmidt, C, Schmid, J, Heinig, LC, Bradtke, P, Rattay, G, Zielinski, S, Hellmig, M, Bartsch, P, Rohde, H, Nuñez, S, Rosemblatt, MV, Rosa Bono, M, Gagliani, N, Sandrock, I, Panzer, U, Krebs, CF, Meyer-Schwesinger, C, Prinz, I & Mittrücker, HW 2023, 'Kidney-resident innate-like memory γδ T cells control chronic Staphylococcus aureus infection of mice', Proceedings of the National Academy of Sciences of the United States of America, vol. 120, no. 1, e2210490120. https://doi.org/10.1073/pnas.2210490120

TY - JOUR

T1 - Kidney-resident innate-like memory γδ T cells control chronic Staphylococcus aureus infection of mice

AU - Bertram, Tabea

AU - Reimers, Daniel

AU - Lory, Niels C.

AU - Schmidt, Constantin

AU - Schmid, Joanna

AU - Heinig, Lisa C.

AU - Bradtke, Peter

AU - Rattay, Guido

AU - Zielinski, Stephanie

AU - Hellmig, Malte

AU - Bartsch, Patricia

AU - Rohde, Holger

AU - Nuñez, Sarah

AU - Rosemblatt, Mariana V.

AU - Rosa Bono, Maria

AU - Gagliani, Nicola

AU - Sandrock, Inga

AU - Panzer, Ulf

AU - Krebs, Christian F.

AU - Meyer-Schwesinger, Catherine

AU - Prinz, Immo

AU - Mittrücker, Hans Willi

PY - 2023/1/3

Y1 - 2023/1/3

N2 - γδ T cells are involved in the control of Staphylococcus aureus infection, but their importance in protection compared to other T cells is unclear. We used a mouse model of systemic S. aureus infection associated with high bacterial load and persistence in the kidney. Infection caused fulminant accumulation of γδ T cells in the kidney. Renal γδ T cells acquired tissue residency and were maintained in high numbers during chronic infection. At day 7, up to 50% of renal γδ T cells produced IL-17A in situ and a large fraction of renal γδ T cells remained IL-17A+ during chronic infection. Controlled depletion revealed that γδ T cells restricted renal S. aureus replication in the acute infection and provided protection during chronic renal infection and upon reinfection. Our results demonstrate that kidney-resident γδ T cells are nonredundant in limiting local S. aureus growth during chronic infection and provide enhanced protection against reinfection.

AB - γδ T cells are involved in the control of Staphylococcus aureus infection, but their importance in protection compared to other T cells is unclear. We used a mouse model of systemic S. aureus infection associated with high bacterial load and persistence in the kidney. Infection caused fulminant accumulation of γδ T cells in the kidney. Renal γδ T cells acquired tissue residency and were maintained in high numbers during chronic infection. At day 7, up to 50% of renal γδ T cells produced IL-17A in situ and a large fraction of renal γδ T cells remained IL-17A+ during chronic infection. Controlled depletion revealed that γδ T cells restricted renal S. aureus replication in the acute infection and provided protection during chronic renal infection and upon reinfection. Our results demonstrate that kidney-resident γδ T cells are nonredundant in limiting local S. aureus growth during chronic infection and provide enhanced protection against reinfection.

KW - IL-17

KW - Staphylococcus aureus

KW - T cell memory

KW - tissue residency

KW - γδ T cells

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U2 - 10.1073/pnas.2210490120

DO - 10.1073/pnas.2210490120

M3 - Article

C2 - 36574651

AN - SCOPUS:85144807448

SN - 0027-8424

VL - 120

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 1

M1 - e2210490120

ER -

Kidney-resident innate-like memory γδ T cells control chronic Staphylococcus aureus infection of mice

Abstract

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