TY - JOUR
T1 - Isolation of an Extensively Drug-Resistant Pseudomonas aeruginosa exoS+/O4 Strain Belonging to the “High-Risk” Clone ST654 and Coproducer of NDM-1 and the Novel VIM-80
AU - Opazo-Capurro, Andrés
AU - Morales-León, Felipe
AU - Jerez, Christian
AU - Olivares-Pacheco, Jorge
AU - Alcalde-Rico, Manuel
AU - González-Muñoz, Paulina
AU - Bello-Toledo, Helia
AU - Cardenas-Arias, Adriana
AU - Esposito, Fernanda
AU - Lincopán, Nilton
AU - Illesca, Vijna
AU - González-Rocha, Gerardo
N1 - Publisher Copyright:
Copyright © 2022 Opazo-Capurro et al.
PY - 2022/9
Y1 - 2022/9
N2 - The aim of this study was to investigate the genomic features of an extensively drug-resistant (XDR) Pseudomonas aeruginosa isolate (P-469) emerging in Chile. Antibiotic susceptibility was determined by disk diffusion and “colistin agar” test. Whole-genome sequencing (WGS) was performed by the Illumina NextSeq 2000 platform, and epidemiologically and clinically relevant data (i.e., sequence-type, serotype, mobile genetic elements, virulome, resistome, plasmidome, prophages, and CRISPR-Cas systems) were retrieved using multiple bioinformatic tools. The P-469 strain displayed an XDR profile, remaining susceptible to colistin. Genomic analysis revealed that this isolate belonged to the “high-risk” clone ST654 (CC654), serotype O4, and genotype exoS+. Strikingly, two CRISPR-Cas systems, five intact prophages sequences, and a broad resistome that included blaNDM-1 and the novel blaVIM-80 carbapenemase genes were predicted. Our results revealed the genomic characteristics of P. aeruginosa belonging to the high-risk clone ST654/O4 coproducing NDM-1 and VIM-80 in Chile, supporting that genomic surveillance is necessary to track the emergence and spread of epidemiologically successful WHO’s critical priority pathogens in order to prevent their rapid dissemination.
AB - The aim of this study was to investigate the genomic features of an extensively drug-resistant (XDR) Pseudomonas aeruginosa isolate (P-469) emerging in Chile. Antibiotic susceptibility was determined by disk diffusion and “colistin agar” test. Whole-genome sequencing (WGS) was performed by the Illumina NextSeq 2000 platform, and epidemiologically and clinically relevant data (i.e., sequence-type, serotype, mobile genetic elements, virulome, resistome, plasmidome, prophages, and CRISPR-Cas systems) were retrieved using multiple bioinformatic tools. The P-469 strain displayed an XDR profile, remaining susceptible to colistin. Genomic analysis revealed that this isolate belonged to the “high-risk” clone ST654 (CC654), serotype O4, and genotype exoS+. Strikingly, two CRISPR-Cas systems, five intact prophages sequences, and a broad resistome that included blaNDM-1 and the novel blaVIM-80 carbapenemase genes were predicted. Our results revealed the genomic characteristics of P. aeruginosa belonging to the high-risk clone ST654/O4 coproducing NDM-1 and VIM-80 in Chile, supporting that genomic surveillance is necessary to track the emergence and spread of epidemiologically successful WHO’s critical priority pathogens in order to prevent their rapid dissemination.
KW - carbapenem-resistant Pseudomonas aeruginosa
KW - high-risk clone
KW - NDM-1
KW - ST654
KW - VIM-80
UR - http://www.scopus.com/inward/record.url?scp=85140856945&partnerID=8YFLogxK
U2 - 10.1128/spectrum.01439-22
DO - 10.1128/spectrum.01439-22
M3 - Article
C2 - 36214677
AN - SCOPUS:85140856945
SN - 2165-0497
VL - 10
JO - Microbiology Spectrum
JF - Microbiology Spectrum
IS - 5
ER -