TY - JOUR
T1 - Intracellular cholesterol deposits associate with mitochondrial dysfunction and lipid storage abnormalities in infertile SR-B1 KO mice
AU - Arias, Andreina
AU - Saavedra, Fujiko
AU - Morselli, Eugenia
AU - Busso, Dolores
PY - 2022/5
Y1 - 2022/5
N2 - Objectives: Female mice deficient in Scavenger Receptor Class B Type I (SR-B1 KO), the main high-density lipoprotein receptor, are infertile. Their oocytes show unesterified cholesterol (UC) excess, high spontaneous activation, and diminished viability. Cholesterol accumulation in different organelles from various cell types impacts their lipid content, viability, and function. Our aim was to characterize cholesterol deposits and global lipid content in eggs from SR-B1 KO females. Method(s): We used eggs from superovulated SR-B1 KO and WT females. Filipin staining (UC marker) was combined with specific organelle markers for mitochondria, lipid droplets (LD), plasma membrane, and lysosomes, and co-localization was determined using Manders' coefficient M2 (n=6 WT and KO oocytes). Mitochondrial membrane potential (MMP) was evaluated using JC-1 dye (n=23 WT and 25 KO oocytes). Lipids were characterized by shotgun lipidomics (3 pools of 100 oocytes/genotype) (Lipotype, Germany). Result(s): In SR-B1 KO eggs, filipin fluorescence in deposits was brighter than in WT eggs [2.3X; p
AB - Objectives: Female mice deficient in Scavenger Receptor Class B Type I (SR-B1 KO), the main high-density lipoprotein receptor, are infertile. Their oocytes show unesterified cholesterol (UC) excess, high spontaneous activation, and diminished viability. Cholesterol accumulation in different organelles from various cell types impacts their lipid content, viability, and function. Our aim was to characterize cholesterol deposits and global lipid content in eggs from SR-B1 KO females. Method(s): We used eggs from superovulated SR-B1 KO and WT females. Filipin staining (UC marker) was combined with specific organelle markers for mitochondria, lipid droplets (LD), plasma membrane, and lysosomes, and co-localization was determined using Manders' coefficient M2 (n=6 WT and KO oocytes). Mitochondrial membrane potential (MMP) was evaluated using JC-1 dye (n=23 WT and 25 KO oocytes). Lipids were characterized by shotgun lipidomics (3 pools of 100 oocytes/genotype) (Lipotype, Germany). Result(s): In SR-B1 KO eggs, filipin fluorescence in deposits was brighter than in WT eggs [2.3X; p
UR - https://www.mendeley.com/catalogue/0a120d12-c2c8-3e15-add1-3d7be51a8a59/
U2 - 10.1016/j.placenta.2022.03.104
DO - 10.1016/j.placenta.2022.03.104
M3 - Artículo
SN - 0143-4004
VL - 122
SP - 27
JO - Placenta
JF - Placenta
ER -