Hantavirus Gn and Gc glycoproteins self-assemble into virus-like particles

Rodrigo Acuña; Nicolás Cifuentes-Muñoz; Chantal L. Márquez; Manuela Bulling; Jonas Klingström; Roberta Mancini; Pierre Yves Lozach; Nicole D. Tischlera

doi:10.1128/JVI.03118-13

Hantavirus Gn and Gc glycoproteins self-assemble into virus-like particles

Rodrigo Acuña, Nicolás Cifuentes-Muñoz, Chantal L. Márquez, Manuela Bulling, Jonas Klingström, Roberta Mancini, Pierre Yves Lozach, Nicole D. Tischlera^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.

Original language	English
Pages (from-to)	2344-2348
Number of pages	5
Journal	Journal of Virology
Volume	88
Issue number	4
DOIs	https://doi.org/10.1128/JVI.03118-13
State	Published - 2014
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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abstract = "How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.",

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AU - Acuña, Rodrigo

AU - Cifuentes-Muñoz, Nicolás

AU - Márquez, Chantal L.

AU - Bulling, Manuela

AU - Klingström, Jonas

AU - Mancini, Roberta

AU - Lozach, Pierre Yves

AU - Tischlera, Nicole D.

PY - 2014/2

Y1 - 2014/2

N2 - How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.

AB - How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.

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JO - Journal of Virology

JF - Journal of Virology

IS - 4

ER -

Hantavirus Gn and Gc glycoproteins self-assemble into virus-like particles

Abstract

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