Fabrication of porous chitosan films impregnated with silver nanoparticles: A facile approach for superior antibacterial application

K. Vimala; Y. Murali Mohan; K. Samba Sivudu; K. Varaprasad; S. Ravindra; N. Narayana Reddy; Y. Padma; B. Sreedhar; K. MohanaRaju

doi:10.1016/j.colsurfb.2009.10.044

Fabrication of porous chitosan films impregnated with silver nanoparticles: A facile approach for superior antibacterial application

K. Vimala, Y. Murali Mohan, K. Samba Sivudu, K. Varaprasad, S. Ravindra, N. Narayana Reddy, Y. Padma, B. Sreedhar, K. MohanaRaju^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

319 Scopus citations

Abstract

The present investigation involves the synthesis of porous chitosan-silver nanocomposite films in view of their increasing areas of application in wound dressing, antibacterial application, and water purification. The entire process consists of three-steps including silver ion-poly(ethylene glycol) matrix preparation, addition of chitosan matrix, and removal of poly(ethylene glycol) from the film matrix. Uniform porous and brown colour chitosan films impregnated with silver nanoparticles (AgNPs) were successfully fabricated by this facile approach. Both, poly(ethylene glycol) (PEG) and chitosan (CS) played vital roles in the reduction of metal ions into nanoparticles (NPs) as well as provided good stability to the formed nanoparticles. The developed porous chitosan-silver nanocomposite (PCSSNC) films were characterized by UV-vis and FTIR spectroscopy, and thermogravimetric analysis for the confirmation of nanoparticles formation. The morphology of silver nanoparticles in nanocomposite films was tested by optical microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The embedded AgNPs were clearly observed throughout the film in SEM and the extracted AgNPs from the porous chitosan-silver nanocomposite showed ∼12 nm in TEM. Improved mechanical properties were observed for porous chitosan-silver nanocomposite than for chitosan blend (CSB) and chitosan-silver nanocomposite (CSSNC) films. Further, the examined antibacterial activity results of these films revealed that porous chitosan-silver nanocomposite films exhibited superior inhibition.

Original language	English
Pages (from-to)	248-258
Number of pages	11
Journal	Colloids and Surfaces B: Biointerfaces
Volume	76
Issue number	1
DOIs	https://doi.org/10.1016/j.colsurfb.2009.10.044
State	Published - 2010
Externally published	Yes

Bibliographical note

Funding Information:
KMR thanks the Defence Research and Development Organization (DRDO) and Ministry of Defence, Government of India, New Delhi and KV thank U.G.C-SAP, New Delhi for the partial financial support.

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

Access to Document

10.1016/j.colsurfb.2009.10.044

Cite this

Vimala, K., Mohan, Y. M., Sivudu, K. S., Varaprasad, K., Ravindra, S., Reddy, N. N., Padma, Y., Sreedhar, B., & MohanaRaju, K. (2010). Fabrication of porous chitosan films impregnated with silver nanoparticles: A facile approach for superior antibacterial application. Colloids and Surfaces B: Biointerfaces, 76(1), 248-258. https://doi.org/10.1016/j.colsurfb.2009.10.044

@article{fd69649a2b3c44bbb16e985b6e338060,

title = "Fabrication of porous chitosan films impregnated with silver nanoparticles: A facile approach for superior antibacterial application",

abstract = "The present investigation involves the synthesis of porous chitosan-silver nanocomposite films in view of their increasing areas of application in wound dressing, antibacterial application, and water purification. The entire process consists of three-steps including silver ion-poly(ethylene glycol) matrix preparation, addition of chitosan matrix, and removal of poly(ethylene glycol) from the film matrix. Uniform porous and brown colour chitosan films impregnated with silver nanoparticles (AgNPs) were successfully fabricated by this facile approach. Both, poly(ethylene glycol) (PEG) and chitosan (CS) played vital roles in the reduction of metal ions into nanoparticles (NPs) as well as provided good stability to the formed nanoparticles. The developed porous chitosan-silver nanocomposite (PCSSNC) films were characterized by UV-vis and FTIR spectroscopy, and thermogravimetric analysis for the confirmation of nanoparticles formation. The morphology of silver nanoparticles in nanocomposite films was tested by optical microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The embedded AgNPs were clearly observed throughout the film in SEM and the extracted AgNPs from the porous chitosan-silver nanocomposite showed ∼12 nm in TEM. Improved mechanical properties were observed for porous chitosan-silver nanocomposite than for chitosan blend (CSB) and chitosan-silver nanocomposite (CSSNC) films. Further, the examined antibacterial activity results of these films revealed that porous chitosan-silver nanocomposite films exhibited superior inhibition.",

keywords = "Antibacterial activity, Chitosan, Hydrogels, Poly(ethylene glycol), Silver nanoparticles, Wound dressing",

author = "K. Vimala and Mohan, {Y. Murali} and Sivudu, {K. Samba} and K. Varaprasad and S. Ravindra and Reddy, {N. Narayana} and Y. Padma and B. Sreedhar and K. MohanaRaju",

note = "Funding Information: KMR thanks the Defence Research and Development Organization (DRDO) and Ministry of Defence, Government of India, New Delhi and KV thank U.G.C-SAP, New Delhi for the partial financial support. ",

year = "2010",

month = mar,

day = "1",

doi = "10.1016/j.colsurfb.2009.10.044",

language = "English",

volume = "76",

pages = "248--258",

journal = "Colloids and Surfaces B: Biointerfaces",

issn = "0927-7765",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Fabrication of porous chitosan films impregnated with silver nanoparticles

T2 - A facile approach for superior antibacterial application

AU - Vimala, K.

AU - Mohan, Y. Murali

AU - Sivudu, K. Samba

AU - Varaprasad, K.

AU - Ravindra, S.

AU - Reddy, N. Narayana

AU - Padma, Y.

AU - Sreedhar, B.

AU - MohanaRaju, K.

N1 - Funding Information: KMR thanks the Defence Research and Development Organization (DRDO) and Ministry of Defence, Government of India, New Delhi and KV thank U.G.C-SAP, New Delhi for the partial financial support.

PY - 2010/3/1

Y1 - 2010/3/1

N2 - The present investigation involves the synthesis of porous chitosan-silver nanocomposite films in view of their increasing areas of application in wound dressing, antibacterial application, and water purification. The entire process consists of three-steps including silver ion-poly(ethylene glycol) matrix preparation, addition of chitosan matrix, and removal of poly(ethylene glycol) from the film matrix. Uniform porous and brown colour chitosan films impregnated with silver nanoparticles (AgNPs) were successfully fabricated by this facile approach. Both, poly(ethylene glycol) (PEG) and chitosan (CS) played vital roles in the reduction of metal ions into nanoparticles (NPs) as well as provided good stability to the formed nanoparticles. The developed porous chitosan-silver nanocomposite (PCSSNC) films were characterized by UV-vis and FTIR spectroscopy, and thermogravimetric analysis for the confirmation of nanoparticles formation. The morphology of silver nanoparticles in nanocomposite films was tested by optical microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The embedded AgNPs were clearly observed throughout the film in SEM and the extracted AgNPs from the porous chitosan-silver nanocomposite showed ∼12 nm in TEM. Improved mechanical properties were observed for porous chitosan-silver nanocomposite than for chitosan blend (CSB) and chitosan-silver nanocomposite (CSSNC) films. Further, the examined antibacterial activity results of these films revealed that porous chitosan-silver nanocomposite films exhibited superior inhibition.

AB - The present investigation involves the synthesis of porous chitosan-silver nanocomposite films in view of their increasing areas of application in wound dressing, antibacterial application, and water purification. The entire process consists of three-steps including silver ion-poly(ethylene glycol) matrix preparation, addition of chitosan matrix, and removal of poly(ethylene glycol) from the film matrix. Uniform porous and brown colour chitosan films impregnated with silver nanoparticles (AgNPs) were successfully fabricated by this facile approach. Both, poly(ethylene glycol) (PEG) and chitosan (CS) played vital roles in the reduction of metal ions into nanoparticles (NPs) as well as provided good stability to the formed nanoparticles. The developed porous chitosan-silver nanocomposite (PCSSNC) films were characterized by UV-vis and FTIR spectroscopy, and thermogravimetric analysis for the confirmation of nanoparticles formation. The morphology of silver nanoparticles in nanocomposite films was tested by optical microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The embedded AgNPs were clearly observed throughout the film in SEM and the extracted AgNPs from the porous chitosan-silver nanocomposite showed ∼12 nm in TEM. Improved mechanical properties were observed for porous chitosan-silver nanocomposite than for chitosan blend (CSB) and chitosan-silver nanocomposite (CSSNC) films. Further, the examined antibacterial activity results of these films revealed that porous chitosan-silver nanocomposite films exhibited superior inhibition.

KW - Antibacterial activity

KW - Chitosan

KW - Hydrogels

KW - Poly(ethylene glycol)

KW - Silver nanoparticles

KW - Wound dressing

UR - http://www.scopus.com/inward/record.url?scp=74149089290&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2009.10.044

DO - 10.1016/j.colsurfb.2009.10.044

M3 - Article

C2 - 19945827

AN - SCOPUS:74149089290

SN - 0927-7765

VL - 76

SP - 248

EP - 258

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

IS - 1

ER -

Fabrication of porous chitosan films impregnated with silver nanoparticles: A facile approach for superior antibacterial application

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this