Expression and subcellular localization of mouse 20S proteasome activator complex PA28

Andrea Soza; Christine Knuehl; Marcus Groettrup; Peter Henklein; Keiji Tanaka; Peter M. Kloetzel

doi:10.1016/S0014-5793(97)00864-8

Expression and subcellular localization of mouse 20S proteasome activator complex PA28

Andrea Soza, Christine Knuehl, Marcus Groettrup, Peter Henklein, Keiji Tanaka, Peter M. Kloetzel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

We have cloned the mouse PA28 proteasome activator cDNAs. Northern blot demonstrates high PA28 mRNA levels in liver, kidney and lung, mRNA levels are low in thymus, spleen and brain. In contrast, PA28 protein levels vary little between these tissues. Immunocytological analysis and cell fractionation experiments demonstrate that both subunits are almost equally distributed between the cytoplasm and the nucleus. Interestingly, PA28α spares nucleoli, while PA28β is strongly enhanced in the nucleolus. This indicates for the first time that the PA28α and PA28β subunits may serve nuclear functions which may be different from and independent of each other.

Original language	English
Pages (from-to)	27-34
Number of pages	8
Journal	FEBS Letters
Volume	413
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(97)00864-8
State	Published - 1997
Externally published	Yes

Bibliographical note

Funding Information:
We like to thank Dr. L. Kühn (Düsseldorf) for the generous gift of antibody hPA28 and purified human PA28. This work was supported by the Deutsche Forschungsgemeinschaft Grant Kl 427/9-1.

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Expression and subcellular localization of mouse 20S proteasome activator complex PA28",

abstract = "We have cloned the mouse PA28 proteasome activator cDNAs. Northern blot demonstrates high PA28 mRNA levels in liver, kidney and lung, mRNA levels are low in thymus, spleen and brain. In contrast, PA28 protein levels vary little between these tissues. Immunocytological analysis and cell fractionation experiments demonstrate that both subunits are almost equally distributed between the cytoplasm and the nucleus. Interestingly, PA28α spares nucleoli, while PA28β is strongly enhanced in the nucleolus. This indicates for the first time that the PA28α and PA28β subunits may serve nuclear functions which may be different from and independent of each other.",

keywords = "Activator, Ki-autoantigen, Proteasome",

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note = "Funding Information: We like to thank Dr. L. K{\"u}hn (D{\"u}sseldorf) for the generous gift of antibody hPA28 and purified human PA28. This work was supported by the Deutsche Forschungsgemeinschaft Grant Kl 427/9-1.",

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doi = "10.1016/S0014-5793(97)00864-8",

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AU - Soza, Andrea

AU - Knuehl, Christine

AU - Groettrup, Marcus

AU - Henklein, Peter

AU - Tanaka, Keiji

AU - Kloetzel, Peter M.

N1 - Funding Information: We like to thank Dr. L. Kühn (Düsseldorf) for the generous gift of antibody hPA28 and purified human PA28. This work was supported by the Deutsche Forschungsgemeinschaft Grant Kl 427/9-1.

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N2 - We have cloned the mouse PA28 proteasome activator cDNAs. Northern blot demonstrates high PA28 mRNA levels in liver, kidney and lung, mRNA levels are low in thymus, spleen and brain. In contrast, PA28 protein levels vary little between these tissues. Immunocytological analysis and cell fractionation experiments demonstrate that both subunits are almost equally distributed between the cytoplasm and the nucleus. Interestingly, PA28α spares nucleoli, while PA28β is strongly enhanced in the nucleolus. This indicates for the first time that the PA28α and PA28β subunits may serve nuclear functions which may be different from and independent of each other.

AB - We have cloned the mouse PA28 proteasome activator cDNAs. Northern blot demonstrates high PA28 mRNA levels in liver, kidney and lung, mRNA levels are low in thymus, spleen and brain. In contrast, PA28 protein levels vary little between these tissues. Immunocytological analysis and cell fractionation experiments demonstrate that both subunits are almost equally distributed between the cytoplasm and the nucleus. Interestingly, PA28α spares nucleoli, while PA28β is strongly enhanced in the nucleolus. This indicates for the first time that the PA28α and PA28β subunits may serve nuclear functions which may be different from and independent of each other.

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Expression and subcellular localization of mouse 20S proteasome activator complex PA28

Abstract

Bibliographical note

ASJC Scopus subject areas

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