TY - JOUR
T1 - Complete sequence of the genome of the human isolate of Andes virus CHI-7913
T2 - Comparative sequence and protein structure analysis
AU - Tischler, Nicole D.
AU - Fernández, Jorge
AU - Müller, Ilse
AU - Martínez, Rodrigo
AU - Galeno, Héctor
AU - Villagra, Eliecer
AU - Mora, Judith
AU - Ramírez, Eugenio
AU - Rosemblatt, Mario
AU - Valenzuela, Pablo D.T.
PY - 2003
Y1 - 2003
N2 - We report here the complete genomic sequence of the Chilean human isolate of Andes virus CHI-7913. The S, M, and L genome segment sequences of this isolate are 1,802, 3,641 and 6,466 bases in length, with an overall GC content of 38.7%. These genome segments code for a nucleocapsid protein of 428 amino acids, a glycoprotein precursor protein of 1,138 amino acids and a RNA-dependent RNA polymerase of 2,152 amino acids. In addition, the genome also has other ORFs coding for putative proteins of 34 to 103 amino acids. The encoded proteins have greater than 98% overall similarity with the proteins of Andes virus isolates AH-1 and Chile R123. Among other sequenced Hantavirus, CHI-7913 is more closely related to Sin Nombre virus, with an overall protein similarity of 92%. The characteristics of the encoded proteins of this isolate, such as hydrophobic domains, glycosylation sites, and conserved amino acid motifs shared with other Hantavirus and other members of the Bunyaviridae family, are identified and discussed.
AB - We report here the complete genomic sequence of the Chilean human isolate of Andes virus CHI-7913. The S, M, and L genome segment sequences of this isolate are 1,802, 3,641 and 6,466 bases in length, with an overall GC content of 38.7%. These genome segments code for a nucleocapsid protein of 428 amino acids, a glycoprotein precursor protein of 1,138 amino acids and a RNA-dependent RNA polymerase of 2,152 amino acids. In addition, the genome also has other ORFs coding for putative proteins of 34 to 103 amino acids. The encoded proteins have greater than 98% overall similarity with the proteins of Andes virus isolates AH-1 and Chile R123. Among other sequenced Hantavirus, CHI-7913 is more closely related to Sin Nombre virus, with an overall protein similarity of 92%. The characteristics of the encoded proteins of this isolate, such as hydrophobic domains, glycosylation sites, and conserved amino acid motifs shared with other Hantavirus and other members of the Bunyaviridae family, are identified and discussed.
KW - Genome sequence
KW - Hantavirus
KW - Human isolate
KW - RNA polymerase
UR - http://www.scopus.com/inward/record.url?scp=10744231536&partnerID=8YFLogxK
U2 - 10.4067/S0716-97602003000200010
DO - 10.4067/S0716-97602003000200010
M3 - Article
C2 - 14513715
AN - SCOPUS:10744231536
SN - 0716-9760
VL - 36
SP - 201
EP - 210
JO - Biological Research
JF - Biological Research
IS - 2
ER -