Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig ClC-5 chloride channel

Isabel Cornejo, María Isabel Niemeyer, Francisco V. Sepúlveda, L. Pablo Cid

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We report the cloning of a guinea pig ClC-5 chloride channel (gpClC-5) from distal small intestinal epithelial cells by RT-PCR and RACE. The transcript is shown to be present in duodenum, jejunum and ileum epithelium by RT-PCR and Northern analysis. This is confirmed by in situ hybridisation which also shows the transcript to be homogeneously distributed in the crypt and villus regions. Expression of gpClC-5 in HEK-293 cells generated markedly outwardly rectified chloride currents with a perm-selectivity sequence of NO3- > Cl- > Br- > I- > F- > gluconate-. The possible role of gpClC-5 in this epithelial location is discussed.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1512
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

Bibliographical note

Funding Information:
Supported by Grant 1990939 from Fondecyt (Chile) and the Volkswagen Stiftung (Germany). We are grateful to Prof. Thomas J. Jentsch for his kind gift of hClC-4 and 5 cDNAs. Institutional support to the Centro de Estudios Cientı́ficos (CECS) from a group of Chilean private companies (Codelco, Dimacofi, Empresas CMPC, Masisa S.A. and Telefónica del Sur) is also acknowledged. The research of F.V.S. was supported in part by an International Research Scholars grant from the Howard Hughes Medical Institute. CECS is a Millennium Science Institute.

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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