Chromogenic plate assay distinguishing bacteriolytic from bacteriostatic activity of an antibiotic agent

Gonzalo Mardones; Alejandro Venegas

doi:10.1016/S0167-7012(00)00125-1

Chromogenic plate assay distinguishing bacteriolytic from bacteriostatic activity of an antibiotic agent

Gonzalo Mardones, Alejandro Venegas

Pontificia Universidad Católica de Chile

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

A solid agar plate assay was devised to discriminate bacteriolytic from bacteriostatic activity for a given antibacterial agent. The assay uses a bacterial culture harboring β-galactosidase enzyme as reporter of cellular lysis. When a drop of bacteriolytic compound is placed on the agar, β-galactosidase is released from the bacteria to the external solid medium where it hydrolyzes X-Gal substrate analogue, developing a blue halo at the edge of the inhibition growth zone. The assay was successfully evaluated against several antibiotics with well-known mechanism of action. It was found that bacteriostatic compounds consistently did not display blue halo at the inhibition zone.

Original language	English
Pages (from-to)	199-206
Number of pages	8
Journal	Journal of Microbiological Methods
Volume	40
Issue number	3
DOIs	https://doi.org/10.1016/S0167-7012(00)00125-1
State	Published - 2000
Externally published	Yes

Bibliographical note

Funding Information:
This research was supported by grants from Fondo Nacional de Ciencia y Tecnologı́a de Chile (FONDECYT #1940713 and #1971010). We gratefully acknowledge Dr Jorge Delgado and Steve Nguyen for critical reading of the manuscript.

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/S0167-7012(00)00125-1

Cite this

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title = "Chromogenic plate assay distinguishing bacteriolytic from bacteriostatic activity of an antibiotic agent",

abstract = "A solid agar plate assay was devised to discriminate bacteriolytic from bacteriostatic activity for a given antibacterial agent. The assay uses a bacterial culture harboring β-galactosidase enzyme as reporter of cellular lysis. When a drop of bacteriolytic compound is placed on the agar, β-galactosidase is released from the bacteria to the external solid medium where it hydrolyzes X-Gal substrate analogue, developing a blue halo at the edge of the inhibition growth zone. The assay was successfully evaluated against several antibiotics with well-known mechanism of action. It was found that bacteriostatic compounds consistently did not display blue halo at the inhibition zone.",

keywords = "Antibiotics, Apidaecin, Bacteriolytic assay, X-Gal plates",

author = "Gonzalo Mardones and Alejandro Venegas",

note = "Funding Information: This research was supported by grants from Fondo Nacional de Ciencia y Tecnolog{\'ı}a de Chile (FONDECYT #1940713 and #1971010). We gratefully acknowledge Dr Jorge Delgado and Steve Nguyen for critical reading of the manuscript.",

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doi = "10.1016/S0167-7012(00)00125-1",

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AU - Mardones, Gonzalo

AU - Venegas, Alejandro

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AB - A solid agar plate assay was devised to discriminate bacteriolytic from bacteriostatic activity for a given antibacterial agent. The assay uses a bacterial culture harboring β-galactosidase enzyme as reporter of cellular lysis. When a drop of bacteriolytic compound is placed on the agar, β-galactosidase is released from the bacteria to the external solid medium where it hydrolyzes X-Gal substrate analogue, developing a blue halo at the edge of the inhibition growth zone. The assay was successfully evaluated against several antibiotics with well-known mechanism of action. It was found that bacteriostatic compounds consistently did not display blue halo at the inhibition zone.

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KW - Apidaecin

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Chromogenic plate assay distinguishing bacteriolytic from bacteriostatic activity of an antibiotic agent

Abstract

Bibliographical note

ASJC Scopus subject areas

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