TY - JOUR
T1 - Biocidal (bacterial and cancer cells) activities of chitosan/CuO nanomaterial, synthesized via a green process
AU - Karthikeyan, Chandrasekaran
AU - Varaprasad, Kokkarachedu
AU - Venugopal, Senthil Kumar
AU - Shakila, S.
AU - Venkatraman, B. R.
AU - Sadiku, Rotimi
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/5/1
Y1 - 2021/5/1
N2 - Biopolymer-based nanomaterials have been developed as antimicrobial and anticancer agents due to their advanced physical, chemical and biomedical characteristics. Herein, chitosan-copper oxide nanomaterial was, successfully synthesized by a green method. In this process, copper salt was nucleated with Psidium guajava leaves extract in order to form the nanomaterial in the chitosan network. Attenuated total reflection-fourier transform, infrared spectroscopy, X-ray diffraction, Dynamic light scattering, Transmission electron microscope, Field emission scanning electron microscopy/Energy dispersive X-ray analysis, X-ray photoelectron spectroscopy and Photoluminescence spectroscopy techniques were, employed to characterize the synthesized nanomaterial. The average size of the nanomaterial was identified to be ∼52.49 nm with XRD. The antibacterial study of CCuO NM showed higher activity than the commercial amoxicillin against gram-positive (G + ve) (Staphylococcus aureus, Bacillus subtilis) and gram-negative (G-ve) bacteria (Klebsiella pneumonia, Escherichia coli). CCuO NM showed in-vitro anticancer potential against human cervical cancer cells (Hela) with an IC50 concentration of 34.69 μg/mL. Photoluminescence spectrum of CCuO NM showed a green emission (oxygen vacancies) observed at ∼516 nm, which is attributed to the generation of reactive oxygen species (ROS) by the nanomaterial, which is believed, to be responsible for the biocidal (cell death) effects. These results suggested that CCuO is a promising nanomaterial that could be suitable for advanced applications in the healthcare industries.
AB - Biopolymer-based nanomaterials have been developed as antimicrobial and anticancer agents due to their advanced physical, chemical and biomedical characteristics. Herein, chitosan-copper oxide nanomaterial was, successfully synthesized by a green method. In this process, copper salt was nucleated with Psidium guajava leaves extract in order to form the nanomaterial in the chitosan network. Attenuated total reflection-fourier transform, infrared spectroscopy, X-ray diffraction, Dynamic light scattering, Transmission electron microscope, Field emission scanning electron microscopy/Energy dispersive X-ray analysis, X-ray photoelectron spectroscopy and Photoluminescence spectroscopy techniques were, employed to characterize the synthesized nanomaterial. The average size of the nanomaterial was identified to be ∼52.49 nm with XRD. The antibacterial study of CCuO NM showed higher activity than the commercial amoxicillin against gram-positive (G + ve) (Staphylococcus aureus, Bacillus subtilis) and gram-negative (G-ve) bacteria (Klebsiella pneumonia, Escherichia coli). CCuO NM showed in-vitro anticancer potential against human cervical cancer cells (Hela) with an IC50 concentration of 34.69 μg/mL. Photoluminescence spectrum of CCuO NM showed a green emission (oxygen vacancies) observed at ∼516 nm, which is attributed to the generation of reactive oxygen species (ROS) by the nanomaterial, which is believed, to be responsible for the biocidal (cell death) effects. These results suggested that CCuO is a promising nanomaterial that could be suitable for advanced applications in the healthcare industries.
KW - Antibacterial and anticancer properties
KW - Chitosan
KW - CuO
KW - Nanomaterial
UR - http://www.scopus.com/inward/record.url?scp=85100764313&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2021.117762
DO - 10.1016/j.carbpol.2021.117762
M3 - Article
C2 - 33674015
AN - SCOPUS:85100764313
SN - 0144-8617
VL - 259
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 117762
ER -
