Andes virus antigens are shed in urine of patients with acute hantavirus cardiopulmonary syndrome

Paula Godoy, Delphine Marsac, Elias Stefas, Pablo Ferrer, Nicole D. Tischler, Karla Pino, Pablo Ramdohr, Pablo Vial, Pablo D.T. Valenzuela, Marcela Ferrés, Francisco Veas, Marcelo López-Lastra*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Hantavirus cardiopulmonary syndrome (HCPS) is a highly pathogenic emerging disease (40% case fatality rate) caused by New World hantaviruses. Hantavirus infections are transmitted to humans mainly by inhalation of virus-contaminated aerosol particles of rodent excreta and secretions. At present, there are no antiviral drugs or immunotherapeutic agents available for the treatment of hantaviral infection, and the survival rates for infected patients hinge largely on early virus recognition and hospital admission and aggressive pulmonary and hemodynamic support. In this study, we show that Andes virus (ANDV) interacts with human apolipoprotein H (ApoH) and that ApoH-coated magnetic beads or ApoH-coated enzyme-linked immunosorbent assay plates can be used to capture and concentrate the virus from complex biological mixtures, such as serum and urine, allowing it to be detected by both immunological and molecular approaches. In addition, we report that ANDV-antigens and infectious virus are shed in urine of HCPS patients.

Original languageEnglish
Pages (from-to)5046-5055
Number of pages10
JournalJournal of Virology
Volume83
Issue number10
DOIs
StatePublished - 2009
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint

Dive into the research topics of 'Andes virus antigens are shed in urine of patients with acute hantavirus cardiopulmonary syndrome'. Together they form a unique fingerprint.

Cite this