TY - JOUR
T1 - Andes virus antigens are shed in urine of patients with acute hantavirus cardiopulmonary syndrome
AU - Godoy, Paula
AU - Marsac, Delphine
AU - Stefas, Elias
AU - Ferrer, Pablo
AU - Tischler, Nicole D.
AU - Pino, Karla
AU - Ramdohr, Pablo
AU - Vial, Pablo
AU - Valenzuela, Pablo D.T.
AU - Ferrés, Marcela
AU - Veas, Francisco
AU - López-Lastra, Marcelo
PY - 2009/5
Y1 - 2009/5
N2 - Hantavirus cardiopulmonary syndrome (HCPS) is a highly pathogenic emerging disease (40% case fatality rate) caused by New World hantaviruses. Hantavirus infections are transmitted to humans mainly by inhalation of virus-contaminated aerosol particles of rodent excreta and secretions. At present, there are no antiviral drugs or immunotherapeutic agents available for the treatment of hantaviral infection, and the survival rates for infected patients hinge largely on early virus recognition and hospital admission and aggressive pulmonary and hemodynamic support. In this study, we show that Andes virus (ANDV) interacts with human apolipoprotein H (ApoH) and that ApoH-coated magnetic beads or ApoH-coated enzyme-linked immunosorbent assay plates can be used to capture and concentrate the virus from complex biological mixtures, such as serum and urine, allowing it to be detected by both immunological and molecular approaches. In addition, we report that ANDV-antigens and infectious virus are shed in urine of HCPS patients.
AB - Hantavirus cardiopulmonary syndrome (HCPS) is a highly pathogenic emerging disease (40% case fatality rate) caused by New World hantaviruses. Hantavirus infections are transmitted to humans mainly by inhalation of virus-contaminated aerosol particles of rodent excreta and secretions. At present, there are no antiviral drugs or immunotherapeutic agents available for the treatment of hantaviral infection, and the survival rates for infected patients hinge largely on early virus recognition and hospital admission and aggressive pulmonary and hemodynamic support. In this study, we show that Andes virus (ANDV) interacts with human apolipoprotein H (ApoH) and that ApoH-coated magnetic beads or ApoH-coated enzyme-linked immunosorbent assay plates can be used to capture and concentrate the virus from complex biological mixtures, such as serum and urine, allowing it to be detected by both immunological and molecular approaches. In addition, we report that ANDV-antigens and infectious virus are shed in urine of HCPS patients.
UR - http://www.scopus.com/inward/record.url?scp=65349149341&partnerID=8YFLogxK
U2 - 10.1128/JVI.02409-08
DO - 10.1128/JVI.02409-08
M3 - Article
C2 - 19279096
AN - SCOPUS:65349149341
SN - 0022-538X
VL - 83
SP - 5046
EP - 5055
JO - Journal of Virology
JF - Journal of Virology
IS - 10
ER -