Analysis of tissue-resident immune cells from mouse skin and lungs by flow cytometry

Felipe Galvez-Cancino; Ernesto Lopez; Alvaro Lladser

doi:10.1007/978-1-4939-8979-9_16

Analysis of tissue-resident immune cells from mouse skin and lungs by flow cytometry

Felipe Galvez-Cancino, Ernesto Lopez, Alvaro Lladser^*

^*Corresponding author for this work

Fundación Ciencia and Vida

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

4 Scopus citations

Abstract

The heterogeneity and complexity of nonlymphoid tissues has become a major obstacle for the study of immune populations. For this reason, the generation of highly reproducible protocols that allow the analysis of immune cells in these tissues has become crucial for clinical and preclinical research. Here we describe an optimized method that allows the obtention of single-cell suspensions from the skin and lungs to analyze and quantify populations of tissue-resident memory CD8 ⁺ T cells by multi-parametric flow cytometry.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	217-222
Number of pages	6
DOIs	https://doi.org/10.1007/978-1-4939-8979-9_16
State	Published - 2019
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1913
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-8979-9_16

Cite this

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abstract = " The heterogeneity and complexity of nonlymphoid tissues has become a major obstacle for the study of immune populations. For this reason, the generation of highly reproducible protocols that allow the analysis of immune cells in these tissues has become crucial for clinical and preclinical research. Here we describe an optimized method that allows the obtention of single-cell suspensions from the skin and lungs to analyze and quantify populations of tissue-resident memory CD8 + T cells by multi-parametric flow cytometry.",

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AU - Lopez, Ernesto

AU - Lladser, Alvaro

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PY - 2019

Y1 - 2019

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AB - The heterogeneity and complexity of nonlymphoid tissues has become a major obstacle for the study of immune populations. For this reason, the generation of highly reproducible protocols that allow the analysis of immune cells in these tissues has become crucial for clinical and preclinical research. Here we describe an optimized method that allows the obtention of single-cell suspensions from the skin and lungs to analyze and quantify populations of tissue-resident memory CD8 + T cells by multi-parametric flow cytometry.

KW - Barrier tissues

KW - Flow cytometry

KW - Immunophenotyping

KW - Memory CD8 T cells

KW - Single-cell suspensions

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PB - Humana Press Inc.

ER -

Analysis of tissue-resident immune cells from mouse skin and lungs by flow cytometry

Abstract

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Bibliographical note

ASJC Scopus subject areas

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